Cloning and sequencing of a carp βs-crystallin cDNA

Tschining Chang; Wen Chang Chang

doi:10.1016/0167-4781(87)90098-4

Cloning and sequencing of a carp β_s-crystallin cDNA

Tschining Chang, Wen Chang Chang

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·10⁷ colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and β_s-crystallins indicates that this carp crystallin sequence resembles closely the bovine β_s-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine β_s-crystallin.

Original language	English
Pages (from-to)	89-92
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	910
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(87)90098-4
Publication status	Published - Oct 9 1987
Externally published	Yes

Keywords

(Carp lens)
cDNA cloning
Nucleotide sequence
β-Crystallin

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/0167-4781(87)90098-4

Cite this

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title = "Cloning and sequencing of a carp βs-crystallin cDNA",

abstract = "The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.",

keywords = "(Carp lens), cDNA cloning, Nucleotide sequence, β-Crystallin",

author = "Tschining Chang and Chang, {Wen Chang}",

year = "1987",

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doi = "10.1016/0167-4781(87)90098-4",

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T1 - Cloning and sequencing of a carp βs-crystallin cDNA

AU - Chang, Tschining

AU - Chang, Wen Chang

PY - 1987/10/9

Y1 - 1987/10/9

N2 - The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.

AB - The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.

KW - (Carp lens)

KW - cDNA cloning

KW - Nucleotide sequence

KW - β-Crystallin

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