TY - JOUR
T1 - Clonal diversity of myelin basic protein-specific T lymphocytes
AU - Huang, Shan Ku
AU - Sriram, Subramaniam
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1988/5
Y1 - 1988/5
N2 - A panel of myelin basic protein (MBP)-specific, class II major histocompatibility complex (As)-restricted T-cell clones were established from SJL/J mice. Three clonotypes, based on their responses to guinea pig MBP and its peptide fragments, were observed. Clonotype I cells, represented by clones HS.6, HS.D2, HS.8, HS.E10, and HS.C1, were reactive to the encephalitogenic C-terminal fragment of MBP, amino acid residues 89-169. Clonotype II, represented by clone HS.E3, was reactive to fragments containing residues 43-88, and clones HS.D12 and HS.C7, representing clonotype III cells, responded to the whole molecule only. Three clones from clonotype I were capable of transferring both clinical and histological signs of experimental allergic encephalomyelitis (EAE) into naive mice. Southern blot analysis of T-cell receptor β-chain genes using Jβ1- and Jβ2-specific probes showed that the rearrangement pattern was unique in each of the clones. These results suggest that the development of EAE may represent an autoaggressive polyclonal T-cell response.
AB - A panel of myelin basic protein (MBP)-specific, class II major histocompatibility complex (As)-restricted T-cell clones were established from SJL/J mice. Three clonotypes, based on their responses to guinea pig MBP and its peptide fragments, were observed. Clonotype I cells, represented by clones HS.6, HS.D2, HS.8, HS.E10, and HS.C1, were reactive to the encephalitogenic C-terminal fragment of MBP, amino acid residues 89-169. Clonotype II, represented by clone HS.E3, was reactive to fragments containing residues 43-88, and clones HS.D12 and HS.C7, representing clonotype III cells, responded to the whole molecule only. Three clones from clonotype I were capable of transferring both clinical and histological signs of experimental allergic encephalomyelitis (EAE) into naive mice. Southern blot analysis of T-cell receptor β-chain genes using Jβ1- and Jβ2-specific probes showed that the rearrangement pattern was unique in each of the clones. These results suggest that the development of EAE may represent an autoaggressive polyclonal T-cell response.
UR - http://www.scopus.com/inward/record.url?scp=0023946193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023946193&partnerID=8YFLogxK
U2 - 10.1007/BF00395133
DO - 10.1007/BF00395133
M3 - Article
C2 - 2451638
AN - SCOPUS:0023946193
SN - 0093-7711
VL - 27
SP - 370
EP - 374
JO - Immunogenetics
JF - Immunogenetics
IS - 5
ER -