Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy

Mei Huei Lin, Young Chau Liu, Shyun Yeu Liu, Fang Chi Chen, Pei Jung Yang, Guan Hua Li, Pang Yen Liu, Ching Yu Yen

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. Results: Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. Conclusions: These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalJournal of Oral Pathology and Medicine
Issue number1
Publication statusPublished - Jan 2018


  • areca nut
  • autophagy
  • benzyl alcohol
  • clathrin
  • dynamin
  • dynasore
  • endocytosis

ASJC Scopus subject areas

  • Oral Surgery
  • Periodontics
  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology


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