Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF: An in vitro and inzovo approach

Chiu Mei Lin; Kou-Gi Shyu; Bao Wei Wang; Hang Chang; Yen Hsu Chen; Jen Hwey Chiu

doi:10.1021/jf100421w

Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF: An in vitro and inzovo approach

Chiu Mei Lin, Kou-Gi Shyu, Bao Wei Wang, Hang Chang, Yen Hsu Chen, Jen Hwey Chiu

Graduate Institute of Injury Prevention and Control

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.

Original language	English
Pages (from-to)	7082-7087
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	58
Issue number	11
DOIs	https://doi.org/10.1021/jf100421w
Publication status	Published - Jun 9 2010

Keywords

Angiogenesis
Chrysin
Gp130/JAK1/STAT3
IL-6
VEGF

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

Access to Document

10.1021/jf100421w

Cite this

@article{0a2ce258f3a24d9682c936372a90f0c5,

title = "Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF: An in vitro and inzovo approach",

abstract = "Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.",

keywords = "Angiogenesis, Chrysin, Gp130/JAK1/STAT3, IL-6, VEGF",

author = "Lin, {Chiu Mei} and Kou-Gi Shyu and Wang, {Bao Wei} and Hang Chang and Chen, {Yen Hsu} and Chiu, {Jen Hwey}",

year = "2010",

month = jun,

day = "9",

doi = "10.1021/jf100421w",

language = "English",

volume = "58",

pages = "7082--7087",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF

T2 - An in vitro and inzovo approach

AU - Lin, Chiu Mei

AU - Shyu, Kou-Gi

AU - Wang, Bao Wei

AU - Chang, Hang

AU - Chen, Yen Hsu

AU - Chiu, Jen Hwey

PY - 2010/6/9

Y1 - 2010/6/9

N2 - Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.

AB - Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.

KW - Angiogenesis

KW - Chrysin

KW - Gp130/JAK1/STAT3

KW - IL-6

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=77953155164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953155164&partnerID=8YFLogxK

U2 - 10.1021/jf100421w

DO - 10.1021/jf100421w

M3 - Article

C2 - 20443595

AN - SCOPUS:77953155164

SN - 0021-8561

VL - 58

SP - 7082

EP - 7087

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 11

ER -

Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF: An in vitro and inzovo approach

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this