TY - JOUR
T1 - Chrysin induces G1 phase cell cycle arrest in C6 glioma cells through inducing p21Waf1/Cip1 expression
T2 - Involvement of p38 mitogen-activated protein kinase
AU - Weng, Meng Shih
AU - Ho, Yuan Soon
AU - Lin, Jen Kun
N1 - Funding Information:
We thank Dr. Yu-Chih Liang for providing excellent technical support. This work was supported by Grants NSC93-2311-B-002-001, 93-2320-B002-111 and NSC93-2320-B-002-127 from the National Science Council.
PY - 2005/6/15
Y1 - 2005/6/15
N2 - Flavonoids are a broadly distributed class of plant pigments, universally present in plants. They are strong anti-oxidants that can inhibit carcinogenesis in rodents. Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound extracted from many plants, honey, and propolis. It possesses potent anti-inflammatory, anti-oxidant properties, promotes cell death, and perturbing cell cycle progression. However, the mechanism by which chrysin inhibits cancer cell growth remains poorly understood. Therefore, we developed an interest in the relationship between MAPK signaling pathways and cell growth inhibition after chrysin treatment in rat C6 glioma cells. Cell viability assay and flow cytometric analysis suggested that chrysin exhibited a dose-dependent and time-dependent ability to block rat C6 glioma cell line cell cycle progression at the G1 phase. Western blotting analysis showed that the levels of Rb phosphorylation in C6 glioma cells exposed to 30 μM chrysin for 24 h decreased significantly. We demonstrated the expression of cyclin-dependent kinase inhibitor, p21Waf1/Cip1, to be significantly increased, but the p53 protein level did not change in chrysin-treated cells. Both cyclin-dependent kinase 2 (CDK2) and 4 (CDK4) kinase activities were reduced by chrysin in a dose-dependent manner. Furthermore, chrysin also inhibited proteasome activity. We further showed that chrysin induced p38-MAPK activation, and using a specific p38-MAPK inhibitor, SB203580, attenuated chrysin-induced p21Waf1/Cip1 expression. These results suggest that chrysin exerts its growth-inhibitory effects either through activating p38-MAPK leading to the accumulation of p21Waf1/Cip1 protein or mediating the inhibition of proteasome activity.
AB - Flavonoids are a broadly distributed class of plant pigments, universally present in plants. They are strong anti-oxidants that can inhibit carcinogenesis in rodents. Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound extracted from many plants, honey, and propolis. It possesses potent anti-inflammatory, anti-oxidant properties, promotes cell death, and perturbing cell cycle progression. However, the mechanism by which chrysin inhibits cancer cell growth remains poorly understood. Therefore, we developed an interest in the relationship between MAPK signaling pathways and cell growth inhibition after chrysin treatment in rat C6 glioma cells. Cell viability assay and flow cytometric analysis suggested that chrysin exhibited a dose-dependent and time-dependent ability to block rat C6 glioma cell line cell cycle progression at the G1 phase. Western blotting analysis showed that the levels of Rb phosphorylation in C6 glioma cells exposed to 30 μM chrysin for 24 h decreased significantly. We demonstrated the expression of cyclin-dependent kinase inhibitor, p21Waf1/Cip1, to be significantly increased, but the p53 protein level did not change in chrysin-treated cells. Both cyclin-dependent kinase 2 (CDK2) and 4 (CDK4) kinase activities were reduced by chrysin in a dose-dependent manner. Furthermore, chrysin also inhibited proteasome activity. We further showed that chrysin induced p38-MAPK activation, and using a specific p38-MAPK inhibitor, SB203580, attenuated chrysin-induced p21Waf1/Cip1 expression. These results suggest that chrysin exerts its growth-inhibitory effects either through activating p38-MAPK leading to the accumulation of p21Waf1/Cip1 protein or mediating the inhibition of proteasome activity.
KW - Cell cycle
KW - Chrysin
KW - Proteasome activity
KW - p21
KW - p38-MAPK
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U2 - 10.1016/j.bcp.2005.03.011
DO - 10.1016/j.bcp.2005.03.011
M3 - Article
C2 - 15869744
AN - SCOPUS:20344388264
SN - 0006-2952
VL - 69
SP - 1815
EP - 1827
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 12
ER -