TY - JOUR
T1 - Chinese herbal medicine SS-1 inhibits T cell activation and abrogates TH responses in Sjögren's syndrome
AU - Lee, Gilbert Aaron
AU - Chang, Ching-Mao
AU - Wu, Ying Chieh
AU - Ma, Ruo-Yu
AU - Chen, Cheng-Yu
AU - Hsue, Yin-Tzu
AU - Liao, Nan-Shih
AU - Chang, Hen-Hong
N1 - Funding Information:
This work was supported by funding from Institute of Molecular Biology, Academia Sinica; China Medical University Hospital (DMR-108-178); Chinese Medicine Research Center, China Medical University (CMRC-CMA-6 from Ministry of Education in Taiwan).
Funding Information:
This work was supported by funding from Institute of Molecular Biology , Academia Sinica ; China Medical University Hospital ( DMR-108-178 ); Chinese Medicine Research Center, China Medical University ( CMRC-CMA-6 from Ministry of Education in Taiwan).
Publisher Copyright:
© 2020
PY - 2021/1
Y1 - 2021/1
N2 - BACKGROUND/PURPOSE: Sjögren's syndrome (SS) is an autoimmune disease and its conventional treatment has exhibited limited therapeutic efficacy. Traditional Chinese medicine has been demonstrated to ameliorate the sicca symptoms of SS by decreasing the level of TH1 and TH2 cytokines and increasing salivary flow rate. A newly designed traditional Chinese medicine, SS-1, showed improved efficacy in alleviating the dryness symptoms of SS patients in the National Taiwan SS cohort investigation. Here, we investigated the effect of SS-1 on T cell responses.METHODS: SS-1 was authenticated and its major compounds were verified by high-performance liquid chromatography. We examined the effects of SS-1 on the activation and TH1, TH2, and TH17 polarization of murine T cells. We also determined the level of TH1, TH2, and TH17 cytokine RNA in peripheral blood mononuclear cells of SS patients before and after SS-1 treatment.RESULTS: SS-1 treatment inhibits the activation and TH1, TH2, and IL-17A+IFNγ+ TH polarization of murine T cells. SS-1 treatment also significantly reduces IFN-γ, IL-4, and IL-13 expression, and moderately reduces IL-17A expression in peripheral blood mononuclear cells of SS patients.CONCLUSION: Our results suggest that SS-1 inhibits T cell activation and diminishes TH1, TH2, and IL-17+IFN-γ+ TH responses in SS patients.
AB - BACKGROUND/PURPOSE: Sjögren's syndrome (SS) is an autoimmune disease and its conventional treatment has exhibited limited therapeutic efficacy. Traditional Chinese medicine has been demonstrated to ameliorate the sicca symptoms of SS by decreasing the level of TH1 and TH2 cytokines and increasing salivary flow rate. A newly designed traditional Chinese medicine, SS-1, showed improved efficacy in alleviating the dryness symptoms of SS patients in the National Taiwan SS cohort investigation. Here, we investigated the effect of SS-1 on T cell responses.METHODS: SS-1 was authenticated and its major compounds were verified by high-performance liquid chromatography. We examined the effects of SS-1 on the activation and TH1, TH2, and TH17 polarization of murine T cells. We also determined the level of TH1, TH2, and TH17 cytokine RNA in peripheral blood mononuclear cells of SS patients before and after SS-1 treatment.RESULTS: SS-1 treatment inhibits the activation and TH1, TH2, and IL-17A+IFNγ+ TH polarization of murine T cells. SS-1 treatment also significantly reduces IFN-γ, IL-4, and IL-13 expression, and moderately reduces IL-17A expression in peripheral blood mononuclear cells of SS patients.CONCLUSION: Our results suggest that SS-1 inhibits T cell activation and diminishes TH1, TH2, and IL-17+IFN-γ+ TH responses in SS patients.
KW - Sjögren's syndrome
KW - T cell activation
KW - T response
KW - Traditional Chinese medicine
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U2 - 10.1016/j.jfma.2020.07.024
DO - 10.1016/j.jfma.2020.07.024
M3 - Article
C2 - 32741737
SN - 0929-6646
VL - 120
SP - 651
EP - 659
JO - Journal of the Formosan Medical Association = Taiwan yi zhi
JF - Journal of the Formosan Medical Association = Taiwan yi zhi
IS - 1 Pt 3
ER -