Characterization of the first clinical isolate of vancomycin-resistant Enterococcus faecalis, AH803, in Taiwan

Jang Jih Lu, Ren Jy Ben, Cherng Lih Perng, Wei Ming Chi, Mong Ling Chu, Wei Hwa Lee

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We previously isolated a vancomycin-resistant strain of Enterococcus faecalis, designated AH803, from the sputum of a patient with pneumonia and bacteremia in Taiwan. AH803 was resistant to vancomycin (minimal inhibitory concentration, MIC = 512 μg/mL) but susceptible to teicoplanin (MIC = 8 μg/mL), and harbored the vanA gene but not the vanB gene. In this study, we further characterized E. faecalis AH803 and the plasmid it was found to contain. DNA from AH803 was analyzed for the presence of vanA and vanB resistance genes by polymerase chain reaction. The vancomycin resistant phenotype was transferable from AH803 to E. faecalis JH2-2, at a frequency of 4.8 x 10-2 AH803 was also resistant to gentamicin and chloramphenicol, and these antibiotic resistance phenotypes cotransferred with vancomycin resistance. The genes responsible for resistance to all three antibiotics were located on a 42-kb conjugative plasmid (pBL101). This plasmid had the same restriction enzyme digestion patterns as Tn 1546, found in piP816 of E. faecalis BM4147. Epidemiologic studies of glycopeptide resistance should perhaps combine phenotypic and genotypic methods, rather than using phenotypic methods alone.

Original languageEnglish
Pages (from-to)178-181
Number of pages4
JournalJournal of the Formosan Medical Association
Issue number2
Publication statusPublished - Feb 2000
Externally publishedYes


  • Conjugative plasmid
  • Enterococcus faecalis AH803
  • Taiwan
  • VanA
  • VanB
  • Vancomycin resistance

ASJC Scopus subject areas

  • General Medicine


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