Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene

Cheng Ming Lee; Yi Ping Shih; Cho Han Wu; Yi Ming Arthur Chen

doi:10.1016/j.gene.2009.05.001

Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene

Cheng Ming Lee, Yi Ping Shih, Cho Han Wu, Yi Ming Arthur Chen

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (- 71/- 67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the - 56/- 47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (- 133/+ 14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the - 104/- 82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.

Original language	English
Pages (from-to)	151-157
Number of pages	7
Journal	Gene
Volume	443
Issue number	1-2
DOIs	https://doi.org/10.1016/j.gene.2009.05.001
Publication status	Published - Aug 15 2009
Externally published	Yes

Keywords

CCAAT box
GNMT
NF-Y
Promoter
Transcriptional start site

ASJC Scopus subject areas

Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.gene.2009.05.001

Cite this

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title = "Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene",

abstract = "Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (- 71/- 67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the - 56/- 47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (- 133/+ 14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the - 104/- 82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.",

keywords = "CCAAT box, GNMT, NF-Y, Promoter, Transcriptional start site",

author = "Lee, {Cheng Ming} and Shih, {Yi Ping} and Wu, {Cho Han} and Chen, {Yi Ming Arthur}",

note = "Funding Information: We thank our colleagues from the AIDS Prevention and Research Center, National Yang-Ming University, for their helpful discussions and technical support, and Mr. Jon Lindemann for his editing assistance. This study was supported in part by grants from the National Research Program on Genomic Medicine of the National Science Council, Republic of China (grant number NSC97-3112-B-010-001) and the National Yang-Ming University Genomic Research Center/Republic of China Ministry of Education (Top University and Center Grant).",

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doi = "10.1016/j.gene.2009.05.001",

language = "English",

volume = "443",

pages = "151--157",

journal = "Gene",

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T1 - Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene

AU - Lee, Cheng Ming

AU - Shih, Yi Ping

AU - Wu, Cho Han

AU - Chen, Yi Ming Arthur

N1 - Funding Information: We thank our colleagues from the AIDS Prevention and Research Center, National Yang-Ming University, for their helpful discussions and technical support, and Mr. Jon Lindemann for his editing assistance. This study was supported in part by grants from the National Research Program on Genomic Medicine of the National Science Council, Republic of China (grant number NSC97-3112-B-010-001) and the National Yang-Ming University Genomic Research Center/Republic of China Ministry of Education (Top University and Center Grant).

PY - 2009/8/15

Y1 - 2009/8/15

N2 - Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (- 71/- 67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the - 56/- 47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (- 133/+ 14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the - 104/- 82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.

AB - Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (- 71/- 67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the - 56/- 47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (- 133/+ 14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the - 104/- 82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.

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KW - Promoter

KW - Transcriptional start site

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Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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