Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Chih Ming Chou; Chang Jen Huang; Chu Fang Lo; Guang Hsung Kou; Chung Hsiung Wang

doi:10.1006/jipa.1996.0041

Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Chih Ming Chou, Chang Jen Huang, Chu Fang Lo, Guang Hsung Kou, Chung Hsiung Wang

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.

Original language	English
Pages (from-to)	259-266
Number of pages	8
Journal	Journal of Invertebrate Pathology
Volume	67
Issue number	3
DOIs	https://doi.org/10.1006/jipa.1996.0041
Publication status	Published - May 1996
Externally published	Yes

Keywords

Baculovirus
Perina nuda nucleopolyhedrovirus
Polyhedrin gene

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1006/jipa.1996.0041

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title = "Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene",

abstract = "The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.",

keywords = "Baculovirus, Perina nuda nucleopolyhedrovirus, Polyhedrin gene",

author = "Chou, {Chih Ming} and Huang, {Chang Jen} and Lo, {Chu Fang} and Kou, {Guang Hsung} and Wang, {Chung Hsiung}",

year = "1996",

month = may,

doi = "10.1006/jipa.1996.0041",

language = "English",

volume = "67",

pages = "259--266",

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T1 - Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

AU - Chou, Chih Ming

AU - Huang, Chang Jen

AU - Lo, Chu Fang

AU - Kou, Guang Hsung

AU - Wang, Chung Hsiung

PY - 1996/5

Y1 - 1996/5

N2 - The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.

AB - The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.

KW - Baculovirus

KW - Perina nuda nucleopolyhedrovirus

KW - Polyhedrin gene

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AN - SCOPUS:0030134452

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JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 3

ER -

Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Abstract

Keywords

ASJC Scopus subject areas

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