TY - JOUR
T1 - Characterization of a novel and potent collagen antagonist, caffeic acid phenethyl ester, in human platelets
T2 - In vitro and in vivo studies
AU - Hsiao, George
AU - Lee, Jie J.
AU - Lin, Kuang H.
AU - Shen, Chia H.
AU - Fong, Tsorng H.
AU - Chou, Duen S.
AU - Sheu, Joen R.
N1 - Funding Information:
This work was supported by grants from the National Science Council of Taiwan (NSC93-2321-B-038-001 and 94-2321-B-038-001) and one from the Topnotch Stroke Research Center Grant, Ministry of Education, Taiwan.
PY - 2007/9/1
Y1 - 2007/9/1
N2 - Objective: Caffeic acid phenethyl ester (CAPE), which is derived from the propolis of honeybee hives, has been demonstrated to possess multiple pharmacological activities. In the present study, CAPE (6-25 μM) specifically inhibited collagen-induced platelet aggregation and the ATP release reaction in platelet suspensions. Methods: Platelet aggregation, flow cytometric analysis, immunoblotting, and electron spin resonance (ESR) were used to assess the anti-platelet activity of CAPE. Fluorescein sodium-induced platelet thrombi in mesenteric microvessels of mice were used for an in vivo study. Results: CAPE (15-100 μM) produced a concentration-related rightward displacement of the collagen concentration-response curve, and the Schild plot gave pA2 and pA10 values of 4.28 ± 0.07 and 3.14 ± 0.73, respectively, with a slope of - 0.83 ± 0.16, indicating specific antagonism. CAPE (25 μM) also inhibited platelet aggregation stimulated by the glycoprotein VI agonist, convulxin, and the α2β1 integrin agonist, aggretin. CAPE (25 μM) also markedly interfered with FITC-collagen binding to platelet membranes. CAPE (15 and 25 μM) concentration-dependently inhibited collagen-induced platelet activation accompanied by [Ca+2]i mobilization, phosphoinositide breakdown, activation of protein kinase C and mitogen-activated protein kinases (i.e., ERK2, JNK, and p38 MAPK), Akt phosphorylation, and thromboxane A2 formation. In the ESR study, CAPE (15 and 25 μM) markedly reduced hydroxyl radical (OH{radical dot}) formation in collagen-activated platelets. In an in vivo study, CAPE (5 mg/kg) significantly prolonged the latency in inducing platelet plug formation in mesenteric venules of mice. Conclusions: The most important findings of this study suggest that CAPE specifically inhibits collagen-induced platelet activation. Thus, CAPE treatment may represent a novel approach to lowering the risk of or improving function in thromboembolism-related disorders.
AB - Objective: Caffeic acid phenethyl ester (CAPE), which is derived from the propolis of honeybee hives, has been demonstrated to possess multiple pharmacological activities. In the present study, CAPE (6-25 μM) specifically inhibited collagen-induced platelet aggregation and the ATP release reaction in platelet suspensions. Methods: Platelet aggregation, flow cytometric analysis, immunoblotting, and electron spin resonance (ESR) were used to assess the anti-platelet activity of CAPE. Fluorescein sodium-induced platelet thrombi in mesenteric microvessels of mice were used for an in vivo study. Results: CAPE (15-100 μM) produced a concentration-related rightward displacement of the collagen concentration-response curve, and the Schild plot gave pA2 and pA10 values of 4.28 ± 0.07 and 3.14 ± 0.73, respectively, with a slope of - 0.83 ± 0.16, indicating specific antagonism. CAPE (25 μM) also inhibited platelet aggregation stimulated by the glycoprotein VI agonist, convulxin, and the α2β1 integrin agonist, aggretin. CAPE (25 μM) also markedly interfered with FITC-collagen binding to platelet membranes. CAPE (15 and 25 μM) concentration-dependently inhibited collagen-induced platelet activation accompanied by [Ca+2]i mobilization, phosphoinositide breakdown, activation of protein kinase C and mitogen-activated protein kinases (i.e., ERK2, JNK, and p38 MAPK), Akt phosphorylation, and thromboxane A2 formation. In the ESR study, CAPE (15 and 25 μM) markedly reduced hydroxyl radical (OH{radical dot}) formation in collagen-activated platelets. In an in vivo study, CAPE (5 mg/kg) significantly prolonged the latency in inducing platelet plug formation in mesenteric venules of mice. Conclusions: The most important findings of this study suggest that CAPE specifically inhibits collagen-induced platelet activation. Thus, CAPE treatment may represent a novel approach to lowering the risk of or improving function in thromboembolism-related disorders.
KW - CAPE
KW - Collagen antagonist
KW - Hydroxyl radical
KW - MAPKs
KW - Platelet activation
UR - http://www.scopus.com/inward/record.url?scp=34547725681&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547725681&partnerID=8YFLogxK
U2 - 10.1016/j.cardiores.2007.05.005
DO - 10.1016/j.cardiores.2007.05.005
M3 - Article
C2 - 17560560
AN - SCOPUS:34547725681
SN - 0008-6363
VL - 75
SP - 782
EP - 792
JO - Cardiovascular Research
JF - Cardiovascular Research
IS - 4
ER -