TY - JOUR
T1 - Changes of superoxide dismutase (SOD) mRNA and activity in response to hypoxic stress in cultured Wistar rat glioma cells
AU - Chang, Cheng Kuei
AU - Tsai, Chung Yau
AU - Lin, Lieh Sheng
AU - Jou, Shuo Bin
AU - Liao, Shue Sen
AU - Cheng, Juei Tang
N1 - Funding Information:
We acknowledge Dr. Ye-Shih Ho for the kind supply of cDNA plasmids. Thanks are also due to Miss S.M. Tai for her excellent technical assistance. The present study is supported in part by a research grant of Mackay Memorial Hospital.
PY - 1997/8/29
Y1 - 1997/8/29
N2 - In an attempt to understand the change of superoxide dismutase (SOD) in tumor cells by hypoxia and hypoxia-normoxia exposure, the present study performed an in vitro investigation using rat glioma cell line in culture. Hypoxia was induced by an incubation with nitrogen gas for 15 h followed the normoxia exposure with air for 30 min. Activity of SOD in cytosolic and particulate of cells was determined by the reduction of nitroblue tetrazolium. Changes of mRNA for Cu,Zn-SOD or Mn-SOD were also characterized using Northern blotting analysis. Hypoxic stress decreased the activity of SOD, both Cu,Zn-SOD and Mn-SOD, in glioma cells. Expression of mRNA for SOD was elevated by hypoxic stress and the increase of mRNA level for Cu,Zn-SOD was more marked than that for Mn-SOD. In response to hypoxia-normoxia exposure, an increase of activity with a lower mRNA level for Mn-SOD was observed in glioma cells. However, changes of Cu,Zn-SOD both the activity and the level of mRNA were not found in glioma cells by hypoxia-normoxia. The obtained results suggest that the SOD in glioma cells can be activated to compensate the damage from free radicals during hypoxic stress.
AB - In an attempt to understand the change of superoxide dismutase (SOD) in tumor cells by hypoxia and hypoxia-normoxia exposure, the present study performed an in vitro investigation using rat glioma cell line in culture. Hypoxia was induced by an incubation with nitrogen gas for 15 h followed the normoxia exposure with air for 30 min. Activity of SOD in cytosolic and particulate of cells was determined by the reduction of nitroblue tetrazolium. Changes of mRNA for Cu,Zn-SOD or Mn-SOD were also characterized using Northern blotting analysis. Hypoxic stress decreased the activity of SOD, both Cu,Zn-SOD and Mn-SOD, in glioma cells. Expression of mRNA for SOD was elevated by hypoxic stress and the increase of mRNA level for Cu,Zn-SOD was more marked than that for Mn-SOD. In response to hypoxia-normoxia exposure, an increase of activity with a lower mRNA level for Mn-SOD was observed in glioma cells. However, changes of Cu,Zn-SOD both the activity and the level of mRNA were not found in glioma cells by hypoxia-normoxia. The obtained results suggest that the SOD in glioma cells can be activated to compensate the damage from free radicals during hypoxic stress.
KW - Enzyme activity and mRNA level
KW - Glioma cell
KW - Hypoxia
KW - Hypoxia and normoxia exposure
KW - Superoxide dismutase
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U2 - 10.1016/S0304-3940(97)00588-0
DO - 10.1016/S0304-3940(97)00588-0
M3 - Article
C2 - 9302100
AN - SCOPUS:0030771398
SN - 0304-3940
VL - 232
SP - 115
EP - 118
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -