Abstract
Background: Oxidative damage plays a central role in atherogenesis and antioxidation defense mechanisms may prevent atherosclerosis. This study evaluated the effect of short-term supplementation of the natural lipophilic antioxidant trilinolein on superoxide dismutase (SOD) activity and SOD-mRNA gene expression in vivo in rat vital organs. Methods: Male Wistar rats (n = 8) were injected intraperitoneally with trilinolein (1 mM/ml/kg/day in 0.5% ethanol) daily for three consecutive days. Two control groups (n = 8) were administered saline or 0.5% ethanol in saline, respectively, for three days. Results: Assay of SOD activity and SOD-mRNA by Northern blotting in rat liver, spleen and brain showed significant increases in SOD activity and increased SOD-mRNA gene expression. Conclusions: The natural lipophilic antioxidant trilinolein potentiates the SOD antioxidation defense mechanism and increases gene expression of SOD-mRNA after short-term supplementation in rats.
Original language | English |
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Pages (from-to) | 355-360 |
Number of pages | 6 |
Journal | Chinese Medical Journal (Taipei) |
Volume | 63 |
Issue number | 5 |
Publication status | Published - May 2000 |
Keywords
- Antioxidant
- Gene expression
- Panax pseudoginseng
- Superoxide dismutase
- Trilinolein
- mRNA
ASJC Scopus subject areas
- General Medicine