Changes in corneal basal epithelial phenotypes in an altered basement membrane

I. Jong Wang, Jui-Fang Tsai, Lung Kun Yeh, Ryan Yao Nien Tsai, Fung Rong Hu, Winston W Y Kao

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.

Original languageEnglish
Article numbere14537
JournalPLoS One
Issue number1
Publication statusPublished - 2011

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Biochemistry,Genetics and Molecular Biology
  • General Medicine


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