Cellular Thiols as a Determinant of Responsiveness to Menadione in Cardiomyocytes

Woan Fang Tzeng, Tzeon Jye Chiou, Chin Peng Wang, Jia Luen Lee, Yee Hsiung Chen

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


The role of intracellular thiols in menadione-mediated toxicity was studied in neonatal rat cardiomyocytes. The sensitivity of cardiomyocytes to menadione was greater than that of skeletal muscle cells and 3T3 fibroblasts. Before cell degeneration, menadione induced marked depletion of intracellular thiols and an increase of oxidized glutathione. The sensitivity of these cells to menadione correlated with the level of depletion of intracellular thiols. After incubation of cardiomyocytes with menadione, glutathione reductase activity was inhibited and lipid peroxidation was increased. Both dicumarol (an inhibitor of DT-diaphorase) and diethyldithiocarbamate (an inhibitor of superoxide dismutase) enhanced the capacity of menadione to induce cellular damage and to cause depletion of intracellular glutathione. Decreasing intracellular glutathione by pretreatment of cells with N-ethylmaleimide or buthionine sulphoximine also increased menadione-induced cell degeneration. Preincubation with cysteine or dithiothreitol suppressed the capacity of menadione to damage the cells. Menadione-induced lipid peroxidation was also suppressed by the same treatment. These results show that the oxidative stress induced by menadione in cardiomyocytes results in the depletion of glutathione and protein thiols. Both DT-diaphorase and superoxide dismutase can protect cells from the toxicity of menadione. Cellular thiols are determinants of the responsiveness to menadione.

Original languageEnglish
Pages (from-to)889-897
Number of pages9
JournalJournal of Molecular and Cellular Cardiology
Issue number7
Publication statusPublished - Jul 1994
Externally publishedYes


  • Cardiomyocytes
  • Glutathione
  • Menadione

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'Cellular Thiols as a Determinant of Responsiveness to Menadione in Cardiomyocytes'. Together they form a unique fingerprint.

Cite this