Cell fractionation on pH-responsive chitosan surface

Yi Hsin Chen, Shao Hsuan Chang, Tsung Jen Wang, I. Jong Wang, Tai Horng Young

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


The purpose of this study is to demonstrate pH-responsive chitosan is able to be used for cell fractionation under precise adjustment of medium pH. Cells were first seeded to attach on chitosan surface at medium pH 7.20 for 24 h. After raising medium pH to 7.65 for 1 h, cells with elongated morphology possessed rapider detachment rate and cells with round shape detached at a lesser rate. Therefore, successful cell separation has been achieved by choosing appropriate cell combination with different detachment rates without additional antibody or enzyme treatment and extensive washing steps. Furthermore, the method also could be applied to specific manipulation of viable cell populations from tissue specimen. Most importantly and interestingly, the efficiency of cell fractionation of our system could be theoretically predicted according to the individual cell detachment rate on pH-responsive chitosan surface, without considering the presence of heterotypic cells.

Original languageEnglish
Pages (from-to)854-863
Number of pages10
Issue number4
Publication statusPublished - Jan 2013


  • Cell detachment
  • Cell fractionation
  • Chitosan
  • PH-responsive

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


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