Abstract
Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.
Original language | English |
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Pages (from-to) | 41-43 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 486 |
DOIs | |
Publication status | Published - Jul 21 2015 |
Keywords
- Chondrogenesis
- DAPI
- Glycosaminoglycan
- Safranin O
- Screening
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology