Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes

Hsin Ying Wu; An Chi Chang; Chia Chi Wang; Fu Hua Kuo; Chi Ya Lee; Der Zen Liu; Tong Rong Jan

doi:10.1016/j.taap.2010.05.003

Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes

Hsin Ying Wu
, An Chi Chang
, Chia Chi Wang
, Fu Hua Kuo
, Chi Ya Lee
, Der Zen Liu
, Tong Rong Jan

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-_L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.

Original language	English
Pages (from-to)	141-147
Number of pages	7
Journal	Toxicology and Applied Pharmacology
Volume	246
Issue number	3
DOIs	https://doi.org/10.1016/j.taap.2010.05.003
Publication status	Published - Aug 2010

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Cannabidiol
Glutathione
Heme oxygenase-1
Monocyte
Thiols

ASJC Scopus subject areas

Toxicology
Pharmacology

Access to Document

10.1016/j.taap.2010.05.003

Cite this

@article{eaca237736204975900970913f23e04c,

title = "Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes",

abstract = "It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.",

keywords = "Apoptosis, Cannabidiol, Glutathione, Heme oxygenase-1, Monocyte, Thiols",

author = "Wu, \{Hsin Ying\} and Chang, \{An Chi\} and Wang, \{Chia Chi\} and Kuo, \{Fu Hua\} and Lee, \{Chi Ya\} and Liu, \{Der Zen\} and Jan, \{Tong Rong\}",

note = "Funding Information: This work was supported by grants NSC98-2923-B-002-001-MY2 and NSC98-2320-B-002-036-MY3 from the National Science Council, Executive Yuan, Taiwan . We also thank Ms. J. S. Wang for her assistance in cell sorting, provided by the Cell Sorting Core Facility (College of Medicine of the National Taiwan University, Taipei, Taiwan). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "2010",

month = aug,

doi = "10.1016/j.taap.2010.05.003",

language = "English",

volume = "246",

pages = "141--147",

journal = "Toxicology and Applied Pharmacology",

issn = "0041-008X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes

AU - Wu, Hsin Ying

AU - Chang, An Chi

AU - Wang, Chia Chi

AU - Kuo, Fu Hua

AU - Lee, Chi Ya

AU - Liu, Der Zen

AU - Jan, Tong Rong

N1 - Funding Information: This work was supported by grants NSC98-2923-B-002-001-MY2 and NSC98-2320-B-002-036-MY3 from the National Science Council, Executive Yuan, Taiwan . We also thank Ms. J. S. Wang for her assistance in cell sorting, provided by the Cell Sorting Core Facility (College of Medicine of the National Taiwan University, Taipei, Taiwan). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 2010/8

Y1 - 2010/8

N2 - It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.

AB - It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.

KW - Apoptosis

KW - Cannabidiol

KW - Glutathione

KW - Heme oxygenase-1

KW - Monocyte

KW - Thiols

UR - https://www.scopus.com/pages/publications/77956876224

UR - https://www.scopus.com/pages/publications/77956876224#tab=citedBy

U2 - 10.1016/j.taap.2010.05.003

DO - 10.1016/j.taap.2010.05.003

M3 - Article

C2 - 20471992

AN - SCOPUS:77956876224

SN - 0041-008X

VL - 246

SP - 141

EP - 147

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

IS - 3

ER -

Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this