Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling

Ying Yu Kuo; Hui Ping Lin; Chieh Huo; Liang Cheng Su; Jonathan Yang; Ping Hsuan Hsiao; Hung Che Chiang; Chi Jung Chung; Horng Dar Wang; Jang Yang Chang; Ya Wen Chen; Chih Pin Chuu

doi:10.3390/ijms14058801

Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling

Ying Yu Kuo, Hui Ping Lin, Chieh Huo, Liang Cheng Su, Jonathan Yang, Ping Hsuan Hsiao, Hung Che Chiang, Chi Jung Chung, Horng Dar Wang, Jang Yang Chang, Ya Wen Chen, Chih Pin Chuu

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27^Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer.

Original language	English
Pages (from-to)	8801-8817
Number of pages	17
Journal	International journal of molecular sciences
Volume	14
Issue number	5
DOIs	https://doi.org/10.3390/ijms14058801
Publication status	Published - May 2013
Externally published	Yes

Keywords

5-fluorouracil
Akt
Akt1
Akt2
Caffeic acid phenethyl ester
Cell cycle
Cell proliferation
Cyclin D1
FOXO1
FOXO3a
NF- κB
Oral cancer
P27
Phospho-Akt Ser473
Phospho-Akt Thr 308
Phospho-FOXO1 Thr24
Phospho-FoxO3a Thr32
Phospho-NF- κB Ser536
Phospho-Rb Ser807/811
Rb
Skp2
TW2.6

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/ijms14058801

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Kuo, Y. Y., Lin, H. P., Huo, C., Su, L. C., Yang, J., Hsiao, P. H., Chiang, H. C., Chung, C. J., Wang, H. D., Chang, J. Y., Chen, Y. W., & Chuu, C. P. (2013). Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling. International journal of molecular sciences, 14(5), 8801-8817. https://doi.org/10.3390/ijms14058801

Kuo, YY, Lin, HP, Huo, C, Su, LC, Yang, J, Hsiao, PH, Chiang, HC, Chung, CJ, Wang, HD, Chang, JY, Chen, YW & Chuu, CP 2013, 'Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling', International journal of molecular sciences, vol. 14, no. 5, pp. 8801-8817. https://doi.org/10.3390/ijms14058801

@article{a1eac64837f7429bb158a2caa831f9ec,

title = "Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling",

abstract = "Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer.",

keywords = "5-fluorouracil, Akt, Akt1, Akt2, Caffeic acid phenethyl ester, Cell cycle, Cell proliferation, Cyclin D1, FOXO1, FOXO3a, NF- κB, Oral cancer, P27, Phospho-Akt Ser473, Phospho-Akt Thr 308, Phospho-FOXO1 Thr24, Phospho-FoxO3a Thr32, Phospho-NF- κB Ser536, Phospho-Rb Ser807/811, Rb, Skp2, TW2.6",

author = "Kuo, {Ying Yu} and Lin, {Hui Ping} and Chieh Huo and Su, {Liang Cheng} and Jonathan Yang and Hsiao, {Ping Hsuan} and Chiang, {Hung Che} and Chung, {Chi Jung} and Wang, {Horng Dar} and Chang, {Jang Yang} and Chen, {Ya Wen} and Chuu, {Chih Pin}",

year = "2013",

month = may,

doi = "10.3390/ijms14058801",

language = "English",

volume = "14",

pages = "8801--8817",

journal = "International journal of molecular sciences",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "5",

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TY - JOUR

T1 - Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling

AU - Kuo, Ying Yu

AU - Lin, Hui Ping

AU - Huo, Chieh

AU - Su, Liang Cheng

AU - Yang, Jonathan

AU - Hsiao, Ping Hsuan

AU - Chiang, Hung Che

AU - Chung, Chi Jung

AU - Wang, Horng Dar

AU - Chang, Jang Yang

AU - Chen, Ya Wen

AU - Chuu, Chih Pin

PY - 2013/5

Y1 - 2013/5

N2 - Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer.

AB - Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer.

KW - 5-fluorouracil

KW - Akt

KW - Akt1

KW - Akt2

KW - Caffeic acid phenethyl ester

KW - Cell cycle

KW - Cell proliferation

KW - Cyclin D1

KW - FOXO1

KW - FOXO3a

KW - NF- κB

KW - Oral cancer

KW - P27

KW - Phospho-Akt Ser473

KW - Phospho-Akt Thr 308

KW - Phospho-FOXO1 Thr24

KW - Phospho-FoxO3a Thr32

KW - Phospho-NF- κB Ser536

KW - Phospho-Rb Ser807/811

KW - Rb

KW - Skp2

KW - TW2.6

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UR - http://www.scopus.com/inward/citedby.url?scp=84876948881&partnerID=8YFLogxK

U2 - 10.3390/ijms14058801

DO - 10.3390/ijms14058801

M3 - Article

C2 - 23615471

AN - SCOPUS:84876948881

SN - 1661-6596

VL - 14

SP - 8801

EP - 8817

JO - International journal of molecular sciences

JF - International journal of molecular sciences

IS - 5

ER -

Caffeic acid phenethyl ester suppresses proliferation and survival of TW2.6 human oral cancer cells via inhibition of akt signaling

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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