Bone healing improvements using hyaluronic acid and hydroxyapatite/beta-Tricalcium phosphate in combination: An animal study

Yen Lan Chang; Yi June Lo; Sheng Wei Feng; Yu Chih Huang; Hsin Yuan Tsai; Che Tong Lin; Kan Hsin Fan; Haw Ming Huang

doi:10.1155/2016/8301624

Bone healing improvements using hyaluronic acid and hydroxyapatite/beta-Tricalcium phosphate in combination: An animal study

Yen Lan Chang, Yi June Lo, Sheng Wei Feng, Yu Chih Huang, Hsin Yuan Tsai, Che Tong Lin, Kan Hsin Fan, Haw Ming Huang

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-Tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0-3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.

Original language	English
Article number	8301624
Journal	BioMed Research International
Volume	2016
DOIs	https://doi.org/10.1155/2016/8301624
Publication status	Published - 2016

ASJC Scopus subject areas

Immunology and Microbiology(all)
Biochemistry, Genetics and Molecular Biology(all)

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title = "Bone healing improvements using hyaluronic acid and hydroxyapatite/beta-Tricalcium phosphate in combination: An animal study",

abstract = "The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-Tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0-3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.",

author = "Chang, {Yen Lan} and Lo, {Yi June} and Feng, {Sheng Wei} and Huang, {Yu Chih} and Tsai, {Hsin Yuan} and Lin, {Che Tong} and Fan, {Kan Hsin} and Huang, {Haw Ming}",

note = "Funding Information: This study was supported by the Ministry of Science and Technology, Taiwan (Grant MOST 104-2221-E-038-002) and in part by Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan (Grant 104TMU-WFH-10). Publisher Copyright: {\textcopyright} 2016 Yen-Lan Chang et al.",

year = "2016",

doi = "10.1155/2016/8301624",

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T1 - Bone healing improvements using hyaluronic acid and hydroxyapatite/beta-Tricalcium phosphate in combination

T2 - An animal study

AU - Chang, Yen Lan

AU - Lo, Yi June

AU - Feng, Sheng Wei

AU - Huang, Yu Chih

AU - Tsai, Hsin Yuan

AU - Lin, Che Tong

AU - Fan, Kan Hsin

AU - Huang, Haw Ming

N1 - Funding Information: This study was supported by the Ministry of Science and Technology, Taiwan (Grant MOST 104-2221-E-038-002) and in part by Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan (Grant 104TMU-WFH-10). Publisher Copyright: © 2016 Yen-Lan Chang et al.

PY - 2016

Y1 - 2016

N2 - The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-Tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0-3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.

AB - The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-Tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0-3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.

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Bone healing improvements using hyaluronic acid and hydroxyapatite/beta-Tricalcium phosphate in combination: An animal study

Abstract

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