Bone defect healing enhanced by ultrasound stimulation: An in vitro tissue culture model

Jui Sheng Sun, Yang Hwei Tsuang, Feng Huei Lin, Hwa Chang Liu, Cheng Zun Tsai, Walter Hong Shong Chang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Ultrasound has many medical applications. Previous animal and clinical studies have clearly shown a positive effect of ultrasound on the rate of osseous repair. The present in vitro study was designed to elucidate the specific response of bony tissue to ultrasound treatment. Bilateral femora were obtained from 36 mature male Wistar rats. A bone defect was created at the center of each distal metaphysis. The femora were maintained for either 7 or 14 days in in vitro tissue culture and received 15 min of ultrasound stimulation or a sham exposure. The ultrasound intensity used was either 320 or 770 mW/cm 2. Healing of the bone defect was evaluated by histomorphological examination and by analysis for the synthesis and secretion of prostaglandin E 2. The results showed that ultrasound stimulation can accelerate both defect healing and trabecular bone regeneration. All experimental femoral defects treated with ultrasound healed faster than the untreated cortical defects, but only the defects receiving 770 mW/cm 2 reached a level that was significantly different. The healing rate for the 320 mW/cm 2 stimulated defects was intermediate between that of the 770-mW/cm 2 and sham-exposed defects. With ultrasound stimulation, prostaglandin E 2 secretion by the experimental femora decreased significantly. Changes in the prostaglandin synthesis and concentration were found to correspond to changes in the amount of trabecular regeneration and to acceleration of bone healing. This highly controlled and well-studied model of ultrasound stimulation of bone healing in vitro can be used to further examine the biological mechanisms involved.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalJournal of Biomedical Materials Research
Issue number2
Publication statusPublished - 1999
Externally publishedYes


  • Bone defect
  • Prostaglandin E
  • Ultrasound stimulation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials


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