TY - JOUR
T1 - Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer
AU - Chuang, Yung Kun
AU - Lai, Szu Hsueh
AU - Lin, Jung Lee
AU - Chen, Chung Hsuan
N1 - Funding Information:
Funding: The authors acknowledge support from the Genomics Research Center, Academia Sinica. We also acknowledge the financial support of the Ministry of Science and Technology (grant NSC 102-2113-M-001-002-MY5) and the National Health Research Institutes (grant NHRI-EX103-10301EI).
Funding Information:
The authors acknowledge support from the Genomics Research Center, Academia Sinica. We also acknowledge the financial support of the Ministry of Science and Technology (grant NSC 102-2113-M-001-002-MY5) and the National Health Research Institutes (grant NHRI-EX103-10301EI).
PY - 2018/9/17
Y1 - 2018/9/17
N2 - We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.
AB - We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.
KW - Bovine serum albumin (BSA)
KW - Cytochrome c
KW - Large clusters
KW - MALDI
KW - Quantitative distribution
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U2 - 10.3390/ijms19092789
DO - 10.3390/ijms19092789
M3 - Article
AN - SCOPUS:85053845997
SN - 1661-6596
VL - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
M1 - 2789
ER -