Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer

Yung Kun Chuang; Szu Hsueh Lai; Jung Lee Lin; Chung Hsuan Chen

doi:10.3390/ijms19092789

Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer

Yung Kun Chuang, Szu Hsueh Lai, Jung Lee Lin, Chung Hsuan Chen

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r² > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.

Original language	English
Article number	2789
Journal	International Journal of Molecular Sciences
Volume	19
Issue number	9
DOIs	https://doi.org/10.3390/ijms19092789
Publication status	Published - Sept 17 2018

Keywords

Bovine serum albumin (BSA)
Cytochrome c
Large clusters
MALDI
Quantitative distribution

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms19092789

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title = "Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer",

abstract = "We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.",

keywords = "Bovine serum albumin (BSA), Cytochrome c, Large clusters, MALDI, Quantitative distribution",

author = "Chuang, {Yung Kun} and Lai, {Szu Hsueh} and Lin, {Jung Lee} and Chen, {Chung Hsuan}",

note = "Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/ijms19092789",

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T1 - Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer

AU - Chuang, Yung Kun

AU - Lai, Szu Hsueh

AU - Lin, Jung Lee

AU - Chen, Chung Hsuan

PY - 2018/9/17

Y1 - 2018/9/17

N2 - We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.

AB - We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.

KW - Bovine serum albumin (BSA)

KW - Cytochrome c

KW - Large clusters

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KW - Quantitative distribution

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Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer

Abstract

Keywords

ASJC Scopus subject areas

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