TY - JOUR
T1 - Biomarkers of DNA damage in patients with end-stage renal disease
T2 - Mitochondrial DNA mutation in hair follicles
AU - Liu, Chin San
AU - Ko, Lie Yon
AU - Lim, Paik Seong
AU - Kao, Shu Huei
AU - Wei, Yau Huei
N1 - Funding Information:
Acknowledgements. This work was supported by a grant (No. NSC88-2314-B010–003) from the National Science Council and partly supported by a grant (DOH88-HR-505) from the National Health Research Institutes, Executive Yuan, Republic of China. Yau-Huei Wei wishes to express his sincere appreciation of the National Science Council and the Medical Research Foundation in Memory of Dr Chi-Shuen Tsou for the generous support of research on mitochondrial DNA mutations in human ageing.
PY - 2001
Y1 - 2001
N2 - Background. DNA damage was noted in patients with end-stage renal disease (ESRD). Mitochondrial DNA (mtDNA) mutations have been proposed as a genomic biomarker in the process of human ageing, degenerative diseases and carcinogenesis. Methods. Polymerase chain reaction (PCR) techniques were applied to detect mtDNA deletions in hair follicles, an appendage of skin, from 162 patients with ESRD. Results. The incidences of the 4977 bp deletion of mtDNA in hair follicles were found to increase with age in normal control and ESRD patients. As compared with normal subjects, ESRD patients had 3.5, 2.3, 2.7, 2.3 and 1.4 times higher incidences of the 4977 bp deletion of mtDNA in the age groups of 20-30, 31-40, 41-50, 51-60 and 61-70 years, respectively. Moreover, the difference in the proportion of mtDNA with the 4977 bp deletion was statistically significant between ESRD patients and normal subjects >50 years of age. Conclusion. We suggest that the 4977 bp deletion of mtDNA in hair follicles may serve as one of the tissue biomarkers of genetic instability of the mitochondrial genome in ESRD patients.
AB - Background. DNA damage was noted in patients with end-stage renal disease (ESRD). Mitochondrial DNA (mtDNA) mutations have been proposed as a genomic biomarker in the process of human ageing, degenerative diseases and carcinogenesis. Methods. Polymerase chain reaction (PCR) techniques were applied to detect mtDNA deletions in hair follicles, an appendage of skin, from 162 patients with ESRD. Results. The incidences of the 4977 bp deletion of mtDNA in hair follicles were found to increase with age in normal control and ESRD patients. As compared with normal subjects, ESRD patients had 3.5, 2.3, 2.7, 2.3 and 1.4 times higher incidences of the 4977 bp deletion of mtDNA in the age groups of 20-30, 31-40, 41-50, 51-60 and 61-70 years, respectively. Moreover, the difference in the proportion of mtDNA with the 4977 bp deletion was statistically significant between ESRD patients and normal subjects >50 years of age. Conclusion. We suggest that the 4977 bp deletion of mtDNA in hair follicles may serve as one of the tissue biomarkers of genetic instability of the mitochondrial genome in ESRD patients.
KW - Biomarker
KW - Deletion
KW - End-stage renal disease
KW - Hair follicles
KW - Mitochondrial DNA
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U2 - 10.1093/ndt/16.3.561
DO - 10.1093/ndt/16.3.561
M3 - Article
C2 - 11239032
AN - SCOPUS:0035090238
SN - 0931-0509
VL - 16
SP - 561
EP - 565
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
IS - 3
ER -
