Biodentine but not MTA induce DSPP expression of dental pulp cells with different severity of LPS-induced inflammation

Min Ching Wang, Kuo Wei Chang, Shu Chun Lin, Pei Shih Hung

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Objectives: To explore the inflammatory and differentiation response in inflamed dental pulp cells (DPCs) induced by lipopolysaccharide (LPS) under different conditions with Biodentine and mineral trioxide aggregate (MTA) treatment. Materials and methods: DPCs were treated with 0.001–1 µg/mL LPS for different periods to induce inflammation. Normal and inflamed DPCs were further treated with 0.14 mg/mL Biodentine or 0.13 mg/mL MTA for different periods. mRNA expression level of IL-6, IL-8 and ALP were analysed by qPCR. DSPP protein expression was detected by western blot. The data were analysed by the Mann–Whitney test, unpaired t test or two-way ANOVA. Results: After treatment for different times and with different concentrations of LPS, different severity of pulp inflammation was revealed by the expressions of IL-6 and IL-8. Higher concentrations of LPS induced higher IL-6 and IL-8 expressions, and these expressions first increased and then decreased (p < 0.0001). At 96 and 192 h, Biodentine significantly suppressed IL-6 expression in both normal and inflamed DPCs (p < 0.05). At 48 and 96 h, Biodentine suppressed ALP expression in both normal and inflamed DPCs (p < 0.05). At 48 and 96 h, Biodentine induced DSPP expressions in both normal and inflamed DPCs (p < 0.05). Conclusion: Biodentine enhanced more DSPP differentiation of both normal and inflamed DPCs under different treatment durations than MTA. Clinical relevance: The prognosis of vital pulp therapy may depend on the severity of pulp inflammation which is difficult to be determined in clinical settings. Therefore, Biodentine may enhance odontogenic differentiation in different severity of pulp inflammation imply its clinical indications.

Original languageEnglish
Pages (from-to)1207-1214
Number of pages8
JournalClinical Oral Investigations
Volume27
Issue number3
DOIs
Publication statusPublished - Mar 2023

Keywords

  • Biodentine
  • Dental pulp cells (DPCs)
  • Inflammation
  • Lipopolysaccharide (LPS)
  • Mineral trioxide aggregate (MTA)
  • Severity

ASJC Scopus subject areas

  • General Dentistry

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