Biodegradable chitosan-chondroitin sulfate sponge for the controlled released of basic fibroblast growth factor

F. L. Mi, Y. B. Wu, P. S. Wang, C. K. Peng, S. S. Shyu, S. H. Yu, M. F. Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution


A new type of chondroitin sulfate (ChS)-chitosan (ChI) polyelectrolyte complex (PEC) sponge for controllable basic fibroblast growth factor (bFGF) release has been prepared by the methods of homogenizing interpolyelectrolyte complex. Since chondroitin sulfate is a recognized bFGF-binding glycosaminoglycan, and is very soluble in water, the ChS-ChI PEC sponges were crosslinked with glutaraldehyde, EDC/NHS and calcium ions respectively to prepare covalent- and ioniccrosslinked polymer networks. The stability, and in vitro enzymatic degradability and cytotoxicity of the glutaraldehyde-, EDC- and Ca2+-crosslinked ChS-ChI PEC sponges were all investigated in this study. The resuls showed that crosslinking improved the stability of prepared ChS-ChI PEC sponges, and provided more protective effect against the dissolution and enzymatic hydrolysis of fixed chondroitin sulfate, as compared to their non-crosslinked counterpart. However, we found that the ionic-crosslinking of ChS-ChI PEC sponges with calcium ions impaired the cell proliferation, suggested that cytotoxicity might be induced by calcium ions. To evaluate the conjugation interaction of bFGF with the ChS-ChI PEC sponges, the effects for the adsorption of bFGF to original and crosslinked-ChS-Chl PEC sponges were examined by ELISA studies. The bFGFconjugated ChS-ChI PEC sponges demonstrated different bFGF release pattern by the variation of crosslinking methods in a concentration-dependent way. The initial burst release could be eliminated due to the ChS-ChI interpolyelectrolyte complex and the crosslinking effect. These results suggest that the modified ChS-ChI PEC sponges may be beneficial to control the bFGF-releasing thus enhances the application potential of the bFGF-conjugated biomaterial for wound repair or tissue engineering.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress


OtherTransactions - 7th World Biomaterials Congress

ASJC Scopus subject areas

  • General Engineering


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