Biocompatible and multifunctional gold nanorods for effective photothermal therapy of oral squamous cell carcinoma

Yu Te Liao; Chia Hung Liu; Yin Chin; Sin Yuan Chen; Shing Hwa Liu; Yih Chih Hsu; Kevin C.W. Wu

doi:10.1039/c9tb00574a

Biocompatible and multifunctional gold nanorods for effective photothermal therapy of oral squamous cell carcinoma

Yu Te Liao, Chia Hung Liu, Yin Chin, Sin Yuan Chen, Shing Hwa Liu, Yih Chih Hsu, Kevin C.W. Wu

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Herein, a cysteine-functionalized alginate was used to replace cytotoxic cetyltrimethylammonium bromide (CTAB) on gold nanorods (GNRs) by gold-thiol bonding. The characterization shows that the alginate-modified GNRs (GNR@Alg-Cys) were 137 nm in length (measured by zetasizer) with a negative surface charge (-28.4 mV). The GNR@Alg-Cys showed an intense absorption at 800 nm, indicating the potential of GNR@Alg-Cys for NIR-excited photothermal therapy (PTT). To target cancer cells, a cyclic peptide, c(RGDfK)KKK, was further modified on GNR@Alg-Cys (denoted as GNR@Alg-Cys/RGD). In vitro experiments reveal the apparently enhanced cell viability under a high dose of GNRs as well as the death of SAS-3 cell lines exposed to a single-wavelength laser (808 nm). The animal study revealed that the volume of GNR@Alg-Cys/RGD-treated laser-exposed tumor was reduced five times when compared with that in the case of the control group (PBS-treated tumor). Furthermore, the in vivo toxicity of GNR@Alg-Cys/RGD was evaluated, and no significant effect was observed on the functions of liver and kidneys after PTT.

Original language	English
Pages (from-to)	4451-4460
Number of pages	10
Journal	Journal of Materials Chemistry B
Volume	7
Issue number	28
DOIs	https://doi.org/10.1039/c9tb00574a
Publication status	Published - 2019

ASJC Scopus subject areas

Chemistry(all)
Biomedical Engineering
Materials Science(all)

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@article{8a75e476b72444bd8dbc1a5ea0341904,

title = "Biocompatible and multifunctional gold nanorods for effective photothermal therapy of oral squamous cell carcinoma",

abstract = "Herein, a cysteine-functionalized alginate was used to replace cytotoxic cetyltrimethylammonium bromide (CTAB) on gold nanorods (GNRs) by gold-thiol bonding. The characterization shows that the alginate-modified GNRs (GNR@Alg-Cys) were 137 nm in length (measured by zetasizer) with a negative surface charge (-28.4 mV). The GNR@Alg-Cys showed an intense absorption at 800 nm, indicating the potential of GNR@Alg-Cys for NIR-excited photothermal therapy (PTT). To target cancer cells, a cyclic peptide, c(RGDfK)KKK, was further modified on GNR@Alg-Cys (denoted as GNR@Alg-Cys/RGD). In vitro experiments reveal the apparently enhanced cell viability under a high dose of GNRs as well as the death of SAS-3 cell lines exposed to a single-wavelength laser (808 nm). The animal study revealed that the volume of GNR@Alg-Cys/RGD-treated laser-exposed tumor was reduced five times when compared with that in the case of the control group (PBS-treated tumor). Furthermore, the in vivo toxicity of GNR@Alg-Cys/RGD was evaluated, and no significant effect was observed on the functions of liver and kidneys after PTT.",

author = "Liao, {Yu Te} and Liu, {Chia Hung} and Yin Chin and Chen, {Sin Yuan} and Liu, {Shing Hwa} and Hsu, {Yih Chih} and Wu, {Kevin C.W.}",

note = "Funding Information: We would like to thank the Ministry of Science and Technology (MOST) of Taiwan (104-2628-E-002-008-MY3; 105-2218-E-155-007; 105-2221-E-002-003-MY3; 105-2221-E-002-227-MY3; 105-2622-E-155-003-CC2; 104-2221-E-033-018-MY3; 106-2119-M-033-002; 107-2119-M-033-001; 107-2221-E-033-006) and the Aim for Top University Project at National Taiwan University (105R7706), Chung Yuan Christian University for the funding support. We also appreciate to Ms C.-Y. Chien (Ministry of Science and Technology) and Mr I-Kuan Lin and Prof. Cheng-Yen Wen (Department of Materials Science and Engineering, National Taiwan University) and for the assistance in TEM experiments. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/c9tb00574a",

language = "English",

volume = "7",

pages = "4451--4460",

journal = "Journal of Materials Chemistry B",

issn = "2050-7518",

publisher = "Royal Society of Chemistry",

number = "28",

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TY - JOUR

T1 - Biocompatible and multifunctional gold nanorods for effective photothermal therapy of oral squamous cell carcinoma

AU - Liao, Yu Te

AU - Liu, Chia Hung

AU - Chin, Yin

AU - Chen, Sin Yuan

AU - Liu, Shing Hwa

AU - Hsu, Yih Chih

AU - Wu, Kevin C.W.

N1 - Funding Information: We would like to thank the Ministry of Science and Technology (MOST) of Taiwan (104-2628-E-002-008-MY3; 105-2218-E-155-007; 105-2221-E-002-003-MY3; 105-2221-E-002-227-MY3; 105-2622-E-155-003-CC2; 104-2221-E-033-018-MY3; 106-2119-M-033-002; 107-2119-M-033-001; 107-2221-E-033-006) and the Aim for Top University Project at National Taiwan University (105R7706), Chung Yuan Christian University for the funding support. We also appreciate to Ms C.-Y. Chien (Ministry of Science and Technology) and Mr I-Kuan Lin and Prof. Cheng-Yen Wen (Department of Materials Science and Engineering, National Taiwan University) and for the assistance in TEM experiments. Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - Herein, a cysteine-functionalized alginate was used to replace cytotoxic cetyltrimethylammonium bromide (CTAB) on gold nanorods (GNRs) by gold-thiol bonding. The characterization shows that the alginate-modified GNRs (GNR@Alg-Cys) were 137 nm in length (measured by zetasizer) with a negative surface charge (-28.4 mV). The GNR@Alg-Cys showed an intense absorption at 800 nm, indicating the potential of GNR@Alg-Cys for NIR-excited photothermal therapy (PTT). To target cancer cells, a cyclic peptide, c(RGDfK)KKK, was further modified on GNR@Alg-Cys (denoted as GNR@Alg-Cys/RGD). In vitro experiments reveal the apparently enhanced cell viability under a high dose of GNRs as well as the death of SAS-3 cell lines exposed to a single-wavelength laser (808 nm). The animal study revealed that the volume of GNR@Alg-Cys/RGD-treated laser-exposed tumor was reduced five times when compared with that in the case of the control group (PBS-treated tumor). Furthermore, the in vivo toxicity of GNR@Alg-Cys/RGD was evaluated, and no significant effect was observed on the functions of liver and kidneys after PTT.

AB - Herein, a cysteine-functionalized alginate was used to replace cytotoxic cetyltrimethylammonium bromide (CTAB) on gold nanorods (GNRs) by gold-thiol bonding. The characterization shows that the alginate-modified GNRs (GNR@Alg-Cys) were 137 nm in length (measured by zetasizer) with a negative surface charge (-28.4 mV). The GNR@Alg-Cys showed an intense absorption at 800 nm, indicating the potential of GNR@Alg-Cys for NIR-excited photothermal therapy (PTT). To target cancer cells, a cyclic peptide, c(RGDfK)KKK, was further modified on GNR@Alg-Cys (denoted as GNR@Alg-Cys/RGD). In vitro experiments reveal the apparently enhanced cell viability under a high dose of GNRs as well as the death of SAS-3 cell lines exposed to a single-wavelength laser (808 nm). The animal study revealed that the volume of GNR@Alg-Cys/RGD-treated laser-exposed tumor was reduced five times when compared with that in the case of the control group (PBS-treated tumor). Furthermore, the in vivo toxicity of GNR@Alg-Cys/RGD was evaluated, and no significant effect was observed on the functions of liver and kidneys after PTT.

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U2 - 10.1039/c9tb00574a

DO - 10.1039/c9tb00574a

M3 - Article

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SN - 2050-7518

VL - 7

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EP - 4460

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 28

ER -

Biocompatible and multifunctional gold nanorods for effective photothermal therapy of oral squamous cell carcinoma

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