Biochemical comparison of γ-crystallins from duck and frog eye lenses

Shyh Horng Chiou, Wen Chang Chang, Jane Kuo, Fu Ming Pan, Tung Bin Lo

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


A biochemical comparison has been made on the crystallins isolated from duck and frog lenses. Gel-permeation chromatography of lens homogenates from both classes on Fractogel TSK HW-55(S) revealed a homogeneous trimeric protein of 120 kDa in the duck lenses and a monomeric protein of 39 kDa in the frog lenses. Both crystallin fractions consist only of an approx. 38-kDa polypeptide in their subunit structures as determined by SDS gel electrophoresis. These two crystalline were compared with respect to their native molecular masses, subunit structures, peptide mapping and amino acid compositions in order to establish the identity of each crystallin. We have found differences in the protein structures of these two crystallins despite some degree of similarity in their amino acid compositions.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalFEBS Letters
Issue number2
Publication statusPublished - Feb 17 1986
Externally publishedYes


  • Duck lens)Amino acid composition Peptide mapping Sequence homology
  • γ-Crystallin(Frog lens

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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