Bio-Kil, a nano-based disinfectant, reduces environmental bacterial burden and multidrug-resistant organisms in intensive care units

Wen Sen Lee, Tai Chin Hsieh, Justine C. Shiau, Tsong Yih Ou, Fu Lun Chen, Yu Hsin Liu, Muh Yong Yen, Po Ren Hsueh

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Background/Purpose This prospective before-after study was intended to investigate the effect of Bio-Kil on reducing environmental bacterial burden and healthcare-associated infections (HAIs) in intensive care units (ICUs) at the Municipal Wan-Fang Hospital, Taipei, Taiwan in 2014. Methods Four rooms in the medical and surgical ICUs were investigated and designated as study rooms (n = 2) or control rooms (n = 2). Routine disinfection was performed during the pre-intervention period in both room types. Bio-Kil was applied to the fomites and surroundings of the study rooms during the intervention period. Total bacterial burden and proportion of colonization of fomites and surroundings by multidrug-resistance organisms (MDROs) were determined before and after the intervention. The demographic characteristics, underlying conditions, and clinical outcomes of patients were analyzed. Results After application of Bio-Kil, the bacterial burden declined in both groups, although the reduction was greater in the study rooms as compared with the control rooms (p = 0.001). During the pre-intervention period, 16 patients were admitted to control rooms and 18 patients to study rooms. After the intervention, 22 patients were admitted to control rooms and 21 patients to study rooms. The number of cases of new-onset sepsis declined in the intervention group (from 33% to 23.8%), but increased in the control group (from 25% to 40.9%); however, there was no significant difference in incidence of new-onset sepsis between the study and control rooms after intervention. Conclusion Application of Bio-Kil reduced the environmental bacterial burden and MDROs in ICUs. Further studies are needed to evaluate the efficacy of this nanotechnology-based disinfectant in reducing HAIs.

Original languageEnglish
Pages (from-to)737-746
Number of pages10
JournalJournal of Microbiology, Immunology and Infection
Issue number5
Publication statusPublished - Oct 2017


  • Bio-Kil
  • environmental decontamination
  • healthcare-associated infections
  • multidrug-resistant organisms

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • Microbiology (medical)
  • Infectious Diseases
  • Immunology and Allergy


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