Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae

Yi Chien Lin; Jing Wen Shih; Chia Ling Hsu; Jing Jer Lin

doi:10.1074/jbc.M104989200

Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae

Yi Chien Lin
, Jing Wen Shih
, Chia Ling Hsu
, Jing Jer Lin

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG_1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG _1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG_1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.

Original language	English
Pages (from-to)	47671-47674
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	276
Issue number	50
DOIs	https://doi.org/10.1074/jbc.M104989200
Publication status	Published - Dec 14 2001
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae",

abstract = "The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.",

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T1 - Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae

AU - Lin, Yi Chien

AU - Shih, Jing Wen

AU - Hsu, Chia Ling

AU - Lin, Jing Jer

PY - 2001/12/14

Y1 - 2001/12/14

N2 - The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.

AB - The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.

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AN - SCOPUS:0035861591

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 50

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Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae

Abstract

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