Bile duct ligation in developing rats: Temporal progression of liver, kidney, and brain damage

Jiunn Ming Sheen, Li Tung Huang, Chih Sung Hsieh, Chih Cheng Chen, Jia Yi Wang, You Lin Tain

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Purpose: Cholestatic liver disease may result in progressive end-stage liver disease and other extrahepatic complications. We explored the temporal progression of bile duct ligation (BDL)-induced cholestasis in developing rats, focusing on brain cognition and liver and kidney pathology, to elucidate whether these findings were associated with asymmetric dimethylarginine and oxidative stress alterations. Materials and Methods: Three groups of young male Sprague-Dawley rats were studied: one group underwent laparotomy (sham), another group underwent laparotomy and BDL for 2 weeks (BDL2), and a third group underwent laparotomy and BDL for 4 weeks (BDL4). Results: The effect of BDL on liver was represented by transforming growth factor β1 levels and histology activity index scores, which were worse in the BDL4 rats than in the BDL2 rats. BDL4 rats also exhibited more severe spatial memory deficits than BDL2 rats. In addition, renal injury was more progressive in BDL4 rats than in BDL2 rats because BDL4 rats displayed higher Cr levels, elevated tubulointerstitial injury scores, neutrophil gelatinase-associated lipocalin, and symmetric dimethylarginine levels. Conclusions: Our findings highlight the fact that young BDL rats exhibit similar trends of progression of liver, kidney, and brain damage. Further studies are needed to better delineate the nature of progression of organ damage in young cholestatic rats.

Original languageEnglish
Pages (from-to)1650-1658
Number of pages9
JournalJournal of Pediatric Surgery
Issue number8
Publication statusPublished - Aug 2010
Externally publishedYes


  • Acute kidney injury
  • Asymmetric dimethylarginine
  • Bile duct ligation
  • Cholestasis, cirrhosis
  • Oxidative stress

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health


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