Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes

Tusty Jiuan Hsieh; Pei Chen Hsieh; Ming Tsang Wu; Wei Chiao Chang; Pi Jung Hsiao; Kun Der Lin; Pong Chun Chou; Shyi Jang Shin

doi:10.1007/s10565-011-9195-5

Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes

Tusty Jiuan Hsieh, Pei Chen Hsieh, Ming Tsang Wu, Wei Chiao Chang, Pi Jung Hsiao, Kun Der Lin, Pong Chun Chou, Shyi Jang Shin

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

According to several population-based studies, betel nut chewing is associated with metabolic syndrome and diabetes in British South Asians and Taiwanese. However, the underlying molecular mechanism is not yet clear. Arecoline is an alkaloid-type natural product found in betel nuts. Our aim was to clarify the influence of betel nut extract and arecoline on lipid accumulation and insulin signaling in adipocytes. We found that betel nut extract and arecoline blocked lipid storage in 3T3-L1 adipocytes. The possible mechanism may function by inhibiting the expression of the insulin receptor, glucose transporter-4, fatty acid synthase, and the lipid droplet proteins perilipin and adipophilin. In addition, betel nut extract and arecoline increased the basal level of IRS-1 serine ³⁰⁷ phosphorylation and decreased insulin-stimulated IRS-1 tyrosine, Akt, and PI3 kinase phosphorylation. In conclusion, betel nut extract and arecoline have diabetogenic potential on adipocytes that may result in insulin resistance and diabetes at least in part via the obstruction of insulin signaling and the blockage of lipid storage.

Original language	English
Pages (from-to)	397-411
Number of pages	15
Journal	Cell Biology and Toxicology
Volume	27
Issue number	6
DOIs	https://doi.org/10.1007/s10565-011-9195-5
Publication status	Published - Dec 2011
Externally published	Yes

Keywords

Betel nut
Diabetes
IRS-1
Insulin resistance
Perilipin

ASJC Scopus subject areas

Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10565-011-9195-5

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title = "Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes",

abstract = "According to several population-based studies, betel nut chewing is associated with metabolic syndrome and diabetes in British South Asians and Taiwanese. However, the underlying molecular mechanism is not yet clear. Arecoline is an alkaloid-type natural product found in betel nuts. Our aim was to clarify the influence of betel nut extract and arecoline on lipid accumulation and insulin signaling in adipocytes. We found that betel nut extract and arecoline blocked lipid storage in 3T3-L1 adipocytes. The possible mechanism may function by inhibiting the expression of the insulin receptor, glucose transporter-4, fatty acid synthase, and the lipid droplet proteins perilipin and adipophilin. In addition, betel nut extract and arecoline increased the basal level of IRS-1 serine 307 phosphorylation and decreased insulin-stimulated IRS-1 tyrosine, Akt, and PI3 kinase phosphorylation. In conclusion, betel nut extract and arecoline have diabetogenic potential on adipocytes that may result in insulin resistance and diabetes at least in part via the obstruction of insulin signaling and the blockage of lipid storage.",

keywords = "Betel nut, Diabetes, IRS-1, Insulin resistance, Perilipin",

author = "Hsieh, {Tusty Jiuan} and Hsieh, {Pei Chen} and Wu, {Ming Tsang} and Chang, {Wei Chiao} and Hsiao, {Pi Jung} and Lin, {Kun Der} and Chou, {Pong Chun} and Shin, {Shyi Jang}",

note = "Funding Information: Acknowledgments This work was supported by grants from the Center of Excellence for Environmental Medicine (KMU-EM-97-1.3.f) and the Medical Research Fund (QM094009 and QM094011) of Kaohsiung Medical University, Taiwan, Republic of China. We also thank Elsevier{\textquoteright}s Webshop for the language editing.",

year = "2011",

month = dec,

doi = "10.1007/s10565-011-9195-5",

language = "English",

volume = "27",

pages = "397--411",

journal = "Cell Biology and Toxicology",

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TY - JOUR

T1 - Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes

AU - Hsieh, Tusty Jiuan

AU - Hsieh, Pei Chen

AU - Wu, Ming Tsang

AU - Chang, Wei Chiao

AU - Hsiao, Pi Jung

AU - Lin, Kun Der

AU - Chou, Pong Chun

AU - Shin, Shyi Jang

N1 - Funding Information: Acknowledgments This work was supported by grants from the Center of Excellence for Environmental Medicine (KMU-EM-97-1.3.f) and the Medical Research Fund (QM094009 and QM094011) of Kaohsiung Medical University, Taiwan, Republic of China. We also thank Elsevier’s Webshop for the language editing.

PY - 2011/12

Y1 - 2011/12

N2 - According to several population-based studies, betel nut chewing is associated with metabolic syndrome and diabetes in British South Asians and Taiwanese. However, the underlying molecular mechanism is not yet clear. Arecoline is an alkaloid-type natural product found in betel nuts. Our aim was to clarify the influence of betel nut extract and arecoline on lipid accumulation and insulin signaling in adipocytes. We found that betel nut extract and arecoline blocked lipid storage in 3T3-L1 adipocytes. The possible mechanism may function by inhibiting the expression of the insulin receptor, glucose transporter-4, fatty acid synthase, and the lipid droplet proteins perilipin and adipophilin. In addition, betel nut extract and arecoline increased the basal level of IRS-1 serine 307 phosphorylation and decreased insulin-stimulated IRS-1 tyrosine, Akt, and PI3 kinase phosphorylation. In conclusion, betel nut extract and arecoline have diabetogenic potential on adipocytes that may result in insulin resistance and diabetes at least in part via the obstruction of insulin signaling and the blockage of lipid storage.

AB - According to several population-based studies, betel nut chewing is associated with metabolic syndrome and diabetes in British South Asians and Taiwanese. However, the underlying molecular mechanism is not yet clear. Arecoline is an alkaloid-type natural product found in betel nuts. Our aim was to clarify the influence of betel nut extract and arecoline on lipid accumulation and insulin signaling in adipocytes. We found that betel nut extract and arecoline blocked lipid storage in 3T3-L1 adipocytes. The possible mechanism may function by inhibiting the expression of the insulin receptor, glucose transporter-4, fatty acid synthase, and the lipid droplet proteins perilipin and adipophilin. In addition, betel nut extract and arecoline increased the basal level of IRS-1 serine 307 phosphorylation and decreased insulin-stimulated IRS-1 tyrosine, Akt, and PI3 kinase phosphorylation. In conclusion, betel nut extract and arecoline have diabetogenic potential on adipocytes that may result in insulin resistance and diabetes at least in part via the obstruction of insulin signaling and the blockage of lipid storage.

KW - Betel nut

KW - Diabetes

KW - IRS-1

KW - Insulin resistance

KW - Perilipin

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Betel nut extract and arecoline block insulin signaling and lipid storage in 3T3-L1 adipocytes

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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