TY - JOUR
T1 - Barcoded microbial system for high-resolution object provenance
AU - Qian, Jason
AU - Lu, Zhi Xiang
AU - Mancuso, Christopher P.
AU - Jhuang, Han Ying
AU - del Carmen Barajas-Ornelas, Rocío
AU - Boswell, Sarah A.
AU - Ramírez-Guadiana, Fernando H.
AU - Jones, Victoria
AU - Sonti, Akhila
AU - Sedlack, Kole
AU - Artzi, Lior
AU - Jung, Giyoung
AU - Arammash, Mohammad
AU - Pettit, Mary E.
AU - Melfi, Michael
AU - Lyon, Lorena
AU - Owen, Siân V.
AU - Baym, Michael
AU - Khalil, Ahmad S.
AU - Silver, Pamela A.
AU - Rudner, David Z.
AU - Springer, Michael
N1 - Publisher Copyright:
© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
PY - 2020/6/5
Y1 - 2020/6/5
N2 - Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment, scalability, rapid and facile decoding, and biocontainment. Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating its implementation in a wide range of applications.
AB - Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment, scalability, rapid and facile decoding, and biocontainment. Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating its implementation in a wide range of applications.
UR - https://www.scopus.com/pages/publications/85085982561
UR - https://www.scopus.com/pages/publications/85085982561#tab=citedBy
U2 - 10.1126/science.aba5584
DO - 10.1126/science.aba5584
M3 - Article
C2 - 32499444
AN - SCOPUS:85085982561
SN - 0036-8075
VL - 368
SP - 1135
EP - 1140
JO - Science
JF - Science
IS - 6495
ER -