Autocrine IL-6 regulates GRO-α production in thymic epithelial cells

Yau Lin Tseng; Ming Ho Wu; Hsin Chiao Yang; Chi Yun Wang; Chiou Feng Lin

doi:10.1016/j.cyto.2010.05.002

Autocrine IL-6 regulates GRO-α production in thymic epithelial cells

Yau Lin Tseng, Ming Ho Wu, Hsin Chiao Yang, Chi Yun Wang, Chiou Feng Lin

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-α (GRO-α) was subsequently expressed after IL-6 production. Either IL-6 or GRO-α was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-α production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-α production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-α and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

Original language	English
Pages (from-to)	195-201
Number of pages	7
Journal	Cytokine
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/j.cyto.2010.05.002
Publication status	Published - Aug 2010
Externally published	Yes

Keywords

GRO-α
IL-6
Jak2
Thymic epithelial cells

ASJC Scopus subject areas

Molecular Biology
Hematology
Biochemistry
Immunology and Allergy
Immunology

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10.1016/j.cyto.2010.05.002

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title = "Autocrine IL-6 regulates GRO-α production in thymic epithelial cells",

abstract = "Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-α (GRO-α) was subsequently expressed after IL-6 production. Either IL-6 or GRO-α was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-α production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-α production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-α and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.",

keywords = "GRO-α, IL-6, Jak2, Thymic epithelial cells",

author = "Tseng, {Yau Lin} and Wu, {Ming Ho} and Yang, {Hsin Chiao} and Wang, {Chi Yun} and Lin, {Chiou Feng}",

year = "2010",

month = aug,

doi = "10.1016/j.cyto.2010.05.002",

language = "English",

volume = "51",

pages = "195--201",

journal = "Cytokine",

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T1 - Autocrine IL-6 regulates GRO-α production in thymic epithelial cells

AU - Tseng, Yau Lin

AU - Wu, Ming Ho

AU - Yang, Hsin Chiao

AU - Wang, Chi Yun

AU - Lin, Chiou Feng

PY - 2010/8

Y1 - 2010/8

N2 - Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-α (GRO-α) was subsequently expressed after IL-6 production. Either IL-6 or GRO-α was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-α production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-α production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-α and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

AB - Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-α (GRO-α) was subsequently expressed after IL-6 production. Either IL-6 or GRO-α was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-α production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-α production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-α and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

KW - GRO-α

KW - IL-6

KW - Jak2

KW - Thymic epithelial cells

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JF - Cytokine

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ER -

Autocrine IL-6 regulates GRO-α production in thymic epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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