Astaxanthin interacts with selenite and attenuates selenite-induced cataractogenesis

Jiahn Haur Liao; Chien Sheng Chen; Timothy J. Maher; Chiung Yueh Liu; Mei Hsiang Lin; Tzu Hua Wu; Shih Hsiung Wu

doi:10.1021/tx800378z

Astaxanthin interacts with selenite and attenuates selenite-induced cataractogenesis

Jiahn Haur Liao, Chien Sheng Chen, Timothy J. Maher, Chiung Yueh Liu, Mei Hsiang Lin, Tzu Hua Wu, Shih Hsiung Wu

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Selenite, the most commonly encountered toxic form of selenium, in overdose, is used to induce cataracts in rats. This study demonstrated that selenite, but not selenate, would interact with the carotenoid astaxanthin (ASTX), as determined using isothermal titration calorimetry and NMR. The maximum absorption of ASTX decreased with increasing selenite concentration, indicating that the conjugated system of ASTX was changed by selenite. Such interactions between ASTX and selenite were also supported by the attenuation of selenite-induced turbidity by ASTX (0-12.5 μM) in vitro. In vivo experiments also showed that ASTX attenuated selenite-induced cataractogenesis in rats. In summary, this is the first report of a direct interaction of ASTX with selenite. This interaction is supported by an in vitro assay and may be partially responsible for the ASTX observed in vivo protection against selenite-induced cataractogenesis.

Original language	English
Pages (from-to)	518-525
Number of pages	8
Journal	Chemical Research in Toxicology
Volume	22
Issue number	3
DOIs	https://doi.org/10.1021/tx800378z
Publication status	Published - Mar 16 2009

ASJC Scopus subject areas

Toxicology

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title = "Astaxanthin interacts with selenite and attenuates selenite-induced cataractogenesis",

abstract = "Selenite, the most commonly encountered toxic form of selenium, in overdose, is used to induce cataracts in rats. This study demonstrated that selenite, but not selenate, would interact with the carotenoid astaxanthin (ASTX), as determined using isothermal titration calorimetry and NMR. The maximum absorption of ASTX decreased with increasing selenite concentration, indicating that the conjugated system of ASTX was changed by selenite. Such interactions between ASTX and selenite were also supported by the attenuation of selenite-induced turbidity by ASTX (0-12.5 μM) in vitro. In vivo experiments also showed that ASTX attenuated selenite-induced cataractogenesis in rats. In summary, this is the first report of a direct interaction of ASTX with selenite. This interaction is supported by an in vitro assay and may be partially responsible for the ASTX observed in vivo protection against selenite-induced cataractogenesis.",

author = "Liao, {Jiahn Haur} and Chen, {Chien Sheng} and Maher, {Timothy J.} and Liu, {Chiung Yueh} and Lin, {Mei Hsiang} and Wu, {Tzu Hua} and Wu, {Shih Hsiung}",

year = "2009",

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doi = "10.1021/tx800378z",

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T1 - Astaxanthin interacts with selenite and attenuates selenite-induced cataractogenesis

AU - Liao, Jiahn Haur

AU - Chen, Chien Sheng

AU - Maher, Timothy J.

AU - Liu, Chiung Yueh

AU - Lin, Mei Hsiang

AU - Wu, Tzu Hua

AU - Wu, Shih Hsiung

PY - 2009/3/16

Y1 - 2009/3/16

N2 - Selenite, the most commonly encountered toxic form of selenium, in overdose, is used to induce cataracts in rats. This study demonstrated that selenite, but not selenate, would interact with the carotenoid astaxanthin (ASTX), as determined using isothermal titration calorimetry and NMR. The maximum absorption of ASTX decreased with increasing selenite concentration, indicating that the conjugated system of ASTX was changed by selenite. Such interactions between ASTX and selenite were also supported by the attenuation of selenite-induced turbidity by ASTX (0-12.5 μM) in vitro. In vivo experiments also showed that ASTX attenuated selenite-induced cataractogenesis in rats. In summary, this is the first report of a direct interaction of ASTX with selenite. This interaction is supported by an in vitro assay and may be partially responsible for the ASTX observed in vivo protection against selenite-induced cataractogenesis.

AB - Selenite, the most commonly encountered toxic form of selenium, in overdose, is used to induce cataracts in rats. This study demonstrated that selenite, but not selenate, would interact with the carotenoid astaxanthin (ASTX), as determined using isothermal titration calorimetry and NMR. The maximum absorption of ASTX decreased with increasing selenite concentration, indicating that the conjugated system of ASTX was changed by selenite. Such interactions between ASTX and selenite were also supported by the attenuation of selenite-induced turbidity by ASTX (0-12.5 μM) in vitro. In vivo experiments also showed that ASTX attenuated selenite-induced cataractogenesis in rats. In summary, this is the first report of a direct interaction of ASTX with selenite. This interaction is supported by an in vitro assay and may be partially responsible for the ASTX observed in vivo protection against selenite-induced cataractogenesis.

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Astaxanthin interacts with selenite and attenuates selenite-induced cataractogenesis

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