Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk

Hsiu Ting Tsai, Ching Hu Wu, Hsiao Ling Lai, Ruei Nian Li, Yi Ching Tung, Hung Yi Chuang, Trong Neng Wu, Li Jen Lin, Chi Kung Ho, Hon Wein Liu, Ming Tsang Wu

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60 Citations (Scopus)


Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or ≥CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95% confidence interval (95% CI), 10.4-99.5] for subjects with CIN1, 37.2 (95% CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95% CI, 14.1-328.5) for subjects with ≥CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P < 0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P < 0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN.

Original languageEnglish
Pages (from-to)2544-2549
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Issue number11 I
Publication statusPublished - Nov 2005
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Oncology


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