Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity

S. Shichijo; N. Keicho; H. T. Long; T. Quy; N. C. Phi; L. D. Ha; V. V. Ban; S. Itoyama; C. J. Hu; N. Komatsu; T. Kirikae; F. Kirikae; S. Shirasawa; M. Kaji; T. Fukuda; M. Sata; T. Kuratsuji; K. Itoh; T. Sasazuki

doi:10.1111/j.1399-0039.2004.00314.x

Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity

S. Shichijo, N. Keicho, H. T. Long, T. Quy, N. C. Phi, L. D. Ha, V. V. Ban, S. Itoyama, C. J. Hu, N. Komatsu, T. Kirikae, F. Kirikae, S. Shirasawa, M. Kaji, T. Fukuda, M. Sata, T. Kuratsuji, K. Itoh, T. Sasazuki

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS-CoV) epitope peptides capable of inducing long-lasting immunity, we first screened immunoglobulin-G (IgG) antibodies reactive to 197 different overlapping 15-mers from the SARS-CoV proteins in the sera of three infected patients. Forty-two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6-month post-infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post-infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long-lasting immunity, may provide a better understanding of the immunogenicity of SARS-CoV.

Original language	English
Pages (from-to)	600-607
Number of pages	8
Journal	Tissue Antigens
Volume	64
Issue number	5
DOIs	https://doi.org/10.1111/j.1399-0039.2004.00314.x
Publication status	Published - Nov 2004
Externally published	Yes

Keywords

IgG
Long-lasting immunity
SARS
Structural protein
Synthetic peptides

ASJC Scopus subject areas

Genetics
Biochemistry
Immunology and Allergy
Immunology

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10.1111/j.1399-0039.2004.00314.x

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Shichijo, S., Keicho, N., Long, H. T., Quy, T., Phi, N. C., Ha, L. D., Ban, V. V., Itoyama, S., Hu, C. J., Komatsu, N., Kirikae, T., Kirikae, F., Shirasawa, S., Kaji, M., Fukuda, T., Sata, M., Kuratsuji, T., Itoh, K., & Sasazuki, T. (2004). Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity. Tissue Antigens, 64(5), 600-607. https://doi.org/10.1111/j.1399-0039.2004.00314.x

Shichijo, S, Keicho, N, Long, HT, Quy, T, Phi, NC, Ha, LD, Ban, VV, Itoyama, S, Hu, CJ, Komatsu, N, Kirikae, T, Kirikae, F, Shirasawa, S, Kaji, M, Fukuda, T, Sata, M, Kuratsuji, T, Itoh, K & Sasazuki, T 2004, 'Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity', Tissue Antigens, vol. 64, no. 5, pp. 600-607. https://doi.org/10.1111/j.1399-0039.2004.00314.x

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abstract = "In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS-CoV) epitope peptides capable of inducing long-lasting immunity, we first screened immunoglobulin-G (IgG) antibodies reactive to 197 different overlapping 15-mers from the SARS-CoV proteins in the sera of three infected patients. Forty-two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6-month post-infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post-infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long-lasting immunity, may provide a better understanding of the immunogenicity of SARS-CoV.",

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AU - Ha, L. D.

AU - Ban, V. V.

AU - Itoyama, S.

AU - Hu, C. J.

AU - Komatsu, N.

AU - Kirikae, T.

AU - Kirikae, F.

AU - Shirasawa, S.

AU - Kaji, M.

AU - Fukuda, T.

AU - Sata, M.

AU - Kuratsuji, T.

AU - Itoh, K.

AU - Sasazuki, T.

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AB - In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS-CoV) epitope peptides capable of inducing long-lasting immunity, we first screened immunoglobulin-G (IgG) antibodies reactive to 197 different overlapping 15-mers from the SARS-CoV proteins in the sera of three infected patients. Forty-two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6-month post-infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post-infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long-lasting immunity, may provide a better understanding of the immunogenicity of SARS-CoV.

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Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity

Abstract

Keywords

ASJC Scopus subject areas

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