Assessment of Early Growth Response 1 in Tumor Suppression of Esophageal Squamous Cell Carcinoma

Yen Chiang Tseng, Chih Wen Shu, Hui Min Chang, Yi Hsuan Lin, Yen Han Tseng, Han Shui Hsu, Yih Gang Goan, Ching Jiunn Tseng

Research output: Contribution to journalArticlepeer-review


Background: Esophageal squamous cell carcinoma (ESCC) is associated with poor survival despite surgical resection, and its pathogenesis has been broadly investigated in the past decade. Early growth response 1 (EGR-1) could involve regulating tumor development in ESCC cells. Methods: An attempt was made to examine the molecular and cellular influence of EGR-1 in esophageal cancer cells by RNA extraction, real-time PCR (qRT-PCR), cell culture, small interfering RNA (siRNA) knockdown, western blot, migration assay, and cell viability assay. One hundred and forty-four samples of ESCC were collected from our hospital and analyzed. Significantly higher EGR-1 expression was noted in tumor-adjacent normal tissue compared with tumor lesions. Results: The univariate analysis showed no significant impacts of EGR-1 expression on patients’ survival. However, after adjusting for the pathological stage, patients with EGR-1 expression > 68th percentile had lower risks of cancer-related death. Moreover, knockdown of EGR-1 significantly enhanced cell migration, invasion, and resistance to chemotherapeutic agents in two ESCC cell lines. Conclusions: EGR-1 plays a key role in tumor suppression involving tumor viability suppression and reflects the treatment effect of current chemotherapy for ESCC.

Original languageEnglish
Article number5792
JournalJournal of Clinical Medicine
Issue number19
Publication statusPublished - Oct 2022
Externally publishedYes


  • EGR-1
  • esophageal carcinoma
  • tumor suppression

ASJC Scopus subject areas

  • Medicine(all)


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