Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells

H. W. Chiu; Y. C. Tseng; Y. J. Wang

doi:10.1201/b16767-188

Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells

H. W. Chiu
, Y. C. Tseng
, Y. J. Wang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Osteosarcoma (HOS cells) and fibrosarcoma (HT1080 cells) were used to investigate the anti-cancer effect of arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that ATO treatment enhanced the percentage of apoptosis and autophagy in HOS and HT1080 cells through increasing the expression of ER stress-associated protein IRE1 and led to induction of LC3-II and cleaved-caspase-3. ATO significantly decreased the phosphorylation of Akt and mTOR and increased the phosphorylation of AMPK, p38, and JNK in HOS and HT1080 cells. Combined treatment of ATO and MG-132 showed synergistic effect through induction of apoptosis and autophagy in HT1080 cells. In in vivo study, the combined treatment significantly reduced the tumor volume in SCID mice that had received a subcutaneous injection of HT1080 cells. Thus, a combination of ATO and proteasome inhibitor could be a new potential therapeutic strategy for the treatment of fibrosarcoma.

Original language	English
Title of host publication	One Century of the Discovery of Arsenicosis in Latin America (1914-2014)
Subtitle of host publication	As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment
Publisher	CRC Press/Balkema
Pages	504-505
Number of pages	2
ISBN (Print)	9781138001411
DOIs	https://doi.org/10.1201/b16767-188
Publication status	Published - 2014
Externally published	Yes
Event	5th International Congress on Arsenic in the Environment, As 2014 - Buenos Aires, Argentina Duration: May 11 2014 → May 16 2014

Publication series

Name	One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment

Other

Other	5th International Congress on Arsenic in the Environment, As 2014
Country/Territory	Argentina
City	Buenos Aires
Period	5/11/14 → 5/16/14

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Chemical Health and Safety

Access to Document

10.1201/b16767-188

Cite this

Chiu, H. W., Tseng, Y. C., & Wang, Y. J. (2014). Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells. In One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment (pp. 504-505). (One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment). CRC Press/Balkema. https://doi.org/10.1201/b16767-188

Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells. / Chiu, H. W.; Tseng, Y. C.; Wang, Y. J.
One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment. CRC Press/Balkema, 2014. p. 504-505 (One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chiu, HW , Tseng, YC & Wang, YJ 2014, Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells. in One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment. One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment, CRC Press/Balkema, pp. 504-505, 5th International Congress on Arsenic in the Environment, As 2014, Buenos Aires, Argentina, 5/11/14. https://doi.org/10.1201/b16767-188

Chiu HW , Tseng YC, Wang YJ. Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells. In One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment. CRC Press/Balkema. 2014. p. 504-505. (One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment). doi: 10.1201/b16767-188

Chiu, H. W. ; Tseng, Y. C. ; Wang, Y. J. / Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells. One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment. CRC Press/Balkema, 2014. pp. 504-505 (One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment).

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abstract = "Osteosarcoma (HOS cells) and fibrosarcoma (HT1080 cells) were used to investigate the anti-cancer effect of arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that ATO treatment enhanced the percentage of apoptosis and autophagy in HOS and HT1080 cells through increasing the expression of ER stress-associated protein IRE1 and led to induction of LC3-II and cleaved-caspase-3. ATO significantly decreased the phosphorylation of Akt and mTOR and increased the phosphorylation of AMPK, p38, and JNK in HOS and HT1080 cells. Combined treatment of ATO and MG-132 showed synergistic effect through induction of apoptosis and autophagy in HT1080 cells. In in vivo study, the combined treatment significantly reduced the tumor volume in SCID mice that had received a subcutaneous injection of HT1080 cells. Thus, a combination of ATO and proteasome inhibitor could be a new potential therapeutic strategy for the treatment of fibrosarcoma.",

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T1 - Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells

AU - Chiu, H. W.

AU - Tseng, Y. C.

AU - Wang, Y. J.

PY - 2014

Y1 - 2014

N2 - Osteosarcoma (HOS cells) and fibrosarcoma (HT1080 cells) were used to investigate the anti-cancer effect of arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that ATO treatment enhanced the percentage of apoptosis and autophagy in HOS and HT1080 cells through increasing the expression of ER stress-associated protein IRE1 and led to induction of LC3-II and cleaved-caspase-3. ATO significantly decreased the phosphorylation of Akt and mTOR and increased the phosphorylation of AMPK, p38, and JNK in HOS and HT1080 cells. Combined treatment of ATO and MG-132 showed synergistic effect through induction of apoptosis and autophagy in HT1080 cells. In in vivo study, the combined treatment significantly reduced the tumor volume in SCID mice that had received a subcutaneous injection of HT1080 cells. Thus, a combination of ATO and proteasome inhibitor could be a new potential therapeutic strategy for the treatment of fibrosarcoma.

AB - Osteosarcoma (HOS cells) and fibrosarcoma (HT1080 cells) were used to investigate the anti-cancer effect of arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that ATO treatment enhanced the percentage of apoptosis and autophagy in HOS and HT1080 cells through increasing the expression of ER stress-associated protein IRE1 and led to induction of LC3-II and cleaved-caspase-3. ATO significantly decreased the phosphorylation of Akt and mTOR and increased the phosphorylation of AMPK, p38, and JNK in HOS and HT1080 cells. Combined treatment of ATO and MG-132 showed synergistic effect through induction of apoptosis and autophagy in HT1080 cells. In in vivo study, the combined treatment significantly reduced the tumor volume in SCID mice that had received a subcutaneous injection of HT1080 cells. Thus, a combination of ATO and proteasome inhibitor could be a new potential therapeutic strategy for the treatment of fibrosarcoma.

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ER -

Arsenic trioxide induces both apoptosis and autophagy through stimulation of ER stress and inhibition of ubiquitin-proteasome system in human sarcoma cells

Abstract

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