Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells

Ying Jan Wang; Hui Wen Chiu; Yi An Chen; Sheng Yow Ho

doi:10.1201/b12522-81

Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells

Ying Jan Wang
, Hui Wen Chiu
, Yi An Chen
, Sheng Yow Ho

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

Original language	English
Title of host publication	Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress
Subtitle of host publication	Arsenic in the Environment
Publisher	Taylor and Francis - Balkema
Pages	206-208
Number of pages	3
ISBN (Print)	9780415637633
DOIs	https://doi.org/10.1201/b12522-81
Publication status	Published - 2012
Externally published	Yes
Event	4th International Congress on Arsenic in the Environment, As 2012 - Cairns, QLD, Australia Duration: Jul 22 2012 → Jul 27 2012

Publication series

Name	Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment

Other

Other	4th International Congress on Arsenic in the Environment, As 2012
Country/Territory	Australia
City	Cairns, QLD
Period	7/22/12 → 7/27/12

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Environmental Chemistry
Pollution

Access to Document

10.1201/b12522-81

Cite this

Wang, Y. J., Chiu, H. W., Chen, Y. A., & Ho, S. Y. (2012). Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment (pp. 206-208). (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment). Taylor and Francis - Balkema. https://doi.org/10.1201/b12522-81

Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. / Wang, Ying Jan; Chiu, Hui Wen ; Chen, Yi An et al.
Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Taylor and Francis - Balkema, 2012. p. 206-208 (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Wang, YJ, Chiu, HW , Chen, YA & Ho, SY 2012, Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. in Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment, Taylor and Francis - Balkema, pp. 206-208, 4th International Congress on Arsenic in the Environment, As 2012, Cairns, QLD, Australia, 7/22/12. https://doi.org/10.1201/b12522-81

Wang YJ, Chiu HW , Chen YA, Ho SY. Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Taylor and Francis - Balkema. 2012. p. 206-208. (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment). doi: 10.1201/b12522-81

Wang, Ying Jan ; Chiu, Hui Wen ; Chen, Yi An et al. / Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Taylor and Francis - Balkema, 2012. pp. 206-208 (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).

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title = "Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells",

abstract = "LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.",

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T1 - Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells

AU - Wang, Ying Jan

AU - Chiu, Hui Wen

AU - Chen, Yi An

AU - Ho, Sheng Yow

PY - 2012

Y1 - 2012

N2 - LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

AB - LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

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DO - 10.1201/b12522-81

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SN - 9780415637633

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BT - Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress

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ER -

Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells

Abstract

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