Armillaria mellea component armillarikin induces apoptosis in human leukemia cells

Yu Jen Chen, Szu-Yuan Wu, Chien Chih Chen, Yu Lin Tsao, Nai Chi Hsu, Yu Chi Chou, Huey Lan Huang

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22 Citations (Scopus)


Honey mushroom Armillaria mellea, a commonly used medicinal food in Asia, is an important component of traditional Chinese medicine "Tien-ma". Armillarikin is a compound isolated from A. mellea with unclear biomedical functions. In this study, we found that armillarikin inhibited the viability of human leukemia K562, U937, and HL-60 cells in a concentration-dependent manner, causing cell death mainly attributable to apoptosis accompanied by reduction of mitochondrial transmembrane potential. Armillarikin induced cleavage of caspase-8, caspase-9, caspase-3, and caspase-3 substrate PARP. Armillarikin-induced growth inhibition and apoptosis were reversed by pan-caspase inhibitor Z-VAD-fmk, indicating a caspase-dependent effect. Moreover, armillarikin increased production of intracellular ROS. Prevention of ROS production attenuated armillarikin-induced apoptosis, suggesting mediation by ROS. In conclusion, armillarikin inhibited growth and induced apoptosis through a mechanism involving mitochondria dysfunction, caspase activation and ROS production in human leukemia K562, U937, and HL-60 cells.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalJournal of Functional Foods
Issue number1
Publication statusPublished - 2014


  • Apoptosis
  • Armillaria mellea
  • Armillarikin
  • Leukemia
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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