Arecoline induces heart injure via Fas/Fas ligand apoptotic pathway in heart of Sprague–Dawley rat

Wen Yuan Lin, Bruce Chi Kang Tsai, Cecilia Hsuan Day, Ping Ling Chiu, Ray Jade Chen, Michael Yu Chih Chen, V. Vijaya Padma, Hsiang Ning Luk, Hsiang Chen Lee, Chih Yang Huang

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Habitual chewing of areca nut increases the risk of cardiovascular disease mortality, but less report demonstrate the toxic mechanism of areca nut on heart. To investigate toxicity of areca nut on cardiomyocytes, we induced the heart injury with arecoline to evaluate the acute damage of areca nut on heart. Different concentrations of are coline (lowdosage: 5 mg/kg/day and high dosage 50 mg/kg/day) were injected into Sprague-Dawley rat via intra-peritoneal method for 21 days to create negative effects of arecoline on cardiomyocyte. Themyocardial architecture of the rat heart was observed. The arecoline-induced apoptotic proteins were analysed via western blotting. The myocardialarchitecture of heart was injured with arecoline and TUNEL stain was also shown are coline-induced cardiac apoptosis. Arecoline promoted the protein expression of both Fas dependent snd mitochondrial dependent apoptosis. In summary, arecoline induces cardiac toxicity and apoptosis by inducing both death receptor and mitochondria-dependent apoptotic pathways on heart.

Original languageEnglish
Pages (from-to)1567-1575
Number of pages9
JournalEnvironmental Toxicology
Issue number8
Publication statusPublished - Aug 2021


  • areca nut
  • arecoline
  • betel nut
  • cardiac toxicity
  • Fas/Fas ligand
  • heart damage

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis


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