Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells

Pin Zhir Chao; Yi Ping Chin; I. Uen Hsu; Cheng-Ming Liu; Ya Chi Yu; Ting-Kai Leung; Yuarn Jang Lee; Chien Ho Chen; Yung Feng Lin

doi:10.1016/j.tiv.2013.05.016

Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells

Pin Zhir Chao, Yi Ping Chin, I. Uen Hsu, Cheng-Ming Liu, Ya Chi Yu, Ting-Kai Leung, Yuarn Jang Lee, Chien Ho Chen, Yung Feng Lin

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.

Original language	English
Pages (from-to)	1870-1876
Number of pages	7
Journal	Toxicology in Vitro
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.tiv.2013.05.016
Publication status	Published - Sept 2013

Keywords

Apoptosis
Death receptor pathway
Destruxin B
Mitochondrial membrane potential
Non-Hodgkin lymphoma

ASJC Scopus subject areas

Toxicology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tiv.2013.05.016

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title = "Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells",

abstract = "Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.",

keywords = "Apoptosis, Death receptor pathway, Destruxin B, Mitochondrial membrane potential, Non-Hodgkin lymphoma",

author = "Chao, {Pin Zhir} and Chin, {Yi Ping} and Hsu, {I. Uen} and Cheng-Ming Liu and Yu, {Ya Chi} and Ting-Kai Leung and Lee, {Yuarn Jang} and Chen, {Chien Ho} and Lin, {Yung Feng}",

year = "2013",

month = sep,

doi = "10.1016/j.tiv.2013.05.016",

language = "English",

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T1 - Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells

AU - Chao, Pin Zhir

AU - Chin, Yi Ping

AU - Hsu, I. Uen

AU - Liu, Cheng-Ming

AU - Yu, Ya Chi

AU - Leung, Ting-Kai

AU - Lee, Yuarn Jang

AU - Chen, Chien Ho

AU - Lin, Yung Feng

PY - 2013/9

Y1 - 2013/9

N2 - Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.

AB - Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.

KW - Apoptosis

KW - Death receptor pathway

KW - Destruxin B

KW - Mitochondrial membrane potential

KW - Non-Hodgkin lymphoma

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Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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