Apoptosis induced by nucleosides in the human hepatoma HepG2

Aim: To investigate the apoptotic effects of nucleosides on the human hepatoma HepG2. Methods: The nucleosides included inosine (I), cytidine (C), uridine (U), thymidine (T), adenosine (A), and guanosine (G). Cells were incubated by the mediums with or without nucleosides at 37 °C in a 50 mL/L CO₂ humidified atmosphere. Results: It was found that the cell viabilities were significantly decreased, when cells were treated with 30 mmol/L I, 30 mmol/L C, 30 mmol/L U, 30 mmol/L T, 0.5 mmol/L A, and 0.5 mmol/L G after 12 h incubation (P1, cells were significantly increased in the mediums containing nucleosides such as C, U, T, A, and G (P