Antioxidative natural product protect against econazole-induced liver injuries

Chi Feng Liu; Chia Hsien Lin; Chun Ching Lin; Yun Ho Lin; Chin Fa Chen; Chao Kuang Lin; Song-Chow Lin

doi:10.1016/j.tox.2003.11.007

Antioxidative natural product protect against econazole-induced liver injuries

Chi Feng Liu, Chia Hsien Lin, Chun Ching Lin, Yun Ho Lin, Chin Fa Chen, Chao Kuang Lin, Song-Chow Lin

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The study objective of this research is in order to investigate the hepatoprotective and therapeutic effects of propolis ethanol extract (PEE) on acute econazole-induced liver injury. Positive control of various concentrations of PEE on liver function and the dose-response relationship of liver injury induced by various doses of econazole were firstly observed from biochemical assay of serum level of aspartate transaminase (SGOT) and serum alanine transaminase (SGPT) and histopathological microscopic examination. The hepatoprotective effects of various concentration of PEE on liver damage induced by hepatotoxic dose (300mg/kg) of econazole were observed by the obvious decrement of SGOT and SGPT level and further confirmed by hepatohistological microscopic examination. The inhibitory effects of PEE on FeCl₂-induced (in vitro) or econazole-induced (in vivo) lipid peroxidation were investigated from the measurement of the formed malonic dialdehyde (MDA) level in the rat liver homogenate. The IC₅₀ (μM) of various concentrations of PEE in the superoxide scavenging activity in econazole (300mg/kg)-damaged rat liver homogenate were assessed by cytochrome c reduction method and compared with that of (+)-alpha-tocopherol. It could be postulated that the hepatoprotective effect of PEE may be, at least in part, due to their inhibitory ability on membrane lipid peroxidation and free radical formation or due to their free radical scavenging ability.

Original language	English
Pages (from-to)	87-93
Number of pages	7
Journal	Toxicology
Volume	196
Issue number	1-2
DOIs	https://doi.org/10.1016/j.tox.2003.11.007
Publication status	Published - Mar 1 2004

Keywords

Cytochrome c
Econazole
Malonic dialdehyde
Propolis ethanol extract

ASJC Scopus subject areas

Toxicology

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10.1016/j.tox.2003.11.007

Cite this

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title = "Antioxidative natural product protect against econazole-induced liver injuries",

abstract = "The study objective of this research is in order to investigate the hepatoprotective and therapeutic effects of propolis ethanol extract (PEE) on acute econazole-induced liver injury. Positive control of various concentrations of PEE on liver function and the dose-response relationship of liver injury induced by various doses of econazole were firstly observed from biochemical assay of serum level of aspartate transaminase (SGOT) and serum alanine transaminase (SGPT) and histopathological microscopic examination. The hepatoprotective effects of various concentration of PEE on liver damage induced by hepatotoxic dose (300mg/kg) of econazole were observed by the obvious decrement of SGOT and SGPT level and further confirmed by hepatohistological microscopic examination. The inhibitory effects of PEE on FeCl2-induced (in vitro) or econazole-induced (in vivo) lipid peroxidation were investigated from the measurement of the formed malonic dialdehyde (MDA) level in the rat liver homogenate. The IC50 (μM) of various concentrations of PEE in the superoxide scavenging activity in econazole (300mg/kg)-damaged rat liver homogenate were assessed by cytochrome c reduction method and compared with that of (+)-alpha-tocopherol. It could be postulated that the hepatoprotective effect of PEE may be, at least in part, due to their inhibitory ability on membrane lipid peroxidation and free radical formation or due to their free radical scavenging ability.",

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author = "Liu, {Chi Feng} and Lin, {Chia Hsien} and Lin, {Chun Ching} and Lin, {Yun Ho} and Chen, {Chin Fa} and Lin, {Chao Kuang} and Song-Chow Lin",

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AU - Liu, Chi Feng

AU - Lin, Chia Hsien

AU - Lin, Chun Ching

AU - Lin, Yun Ho

AU - Chen, Chin Fa

AU - Lin, Chao Kuang

AU - Lin, Song-Chow

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N2 - The study objective of this research is in order to investigate the hepatoprotective and therapeutic effects of propolis ethanol extract (PEE) on acute econazole-induced liver injury. Positive control of various concentrations of PEE on liver function and the dose-response relationship of liver injury induced by various doses of econazole were firstly observed from biochemical assay of serum level of aspartate transaminase (SGOT) and serum alanine transaminase (SGPT) and histopathological microscopic examination. The hepatoprotective effects of various concentration of PEE on liver damage induced by hepatotoxic dose (300mg/kg) of econazole were observed by the obvious decrement of SGOT and SGPT level and further confirmed by hepatohistological microscopic examination. The inhibitory effects of PEE on FeCl2-induced (in vitro) or econazole-induced (in vivo) lipid peroxidation were investigated from the measurement of the formed malonic dialdehyde (MDA) level in the rat liver homogenate. The IC50 (μM) of various concentrations of PEE in the superoxide scavenging activity in econazole (300mg/kg)-damaged rat liver homogenate were assessed by cytochrome c reduction method and compared with that of (+)-alpha-tocopherol. It could be postulated that the hepatoprotective effect of PEE may be, at least in part, due to their inhibitory ability on membrane lipid peroxidation and free radical formation or due to their free radical scavenging ability.

AB - The study objective of this research is in order to investigate the hepatoprotective and therapeutic effects of propolis ethanol extract (PEE) on acute econazole-induced liver injury. Positive control of various concentrations of PEE on liver function and the dose-response relationship of liver injury induced by various doses of econazole were firstly observed from biochemical assay of serum level of aspartate transaminase (SGOT) and serum alanine transaminase (SGPT) and histopathological microscopic examination. The hepatoprotective effects of various concentration of PEE on liver damage induced by hepatotoxic dose (300mg/kg) of econazole were observed by the obvious decrement of SGOT and SGPT level and further confirmed by hepatohistological microscopic examination. The inhibitory effects of PEE on FeCl2-induced (in vitro) or econazole-induced (in vivo) lipid peroxidation were investigated from the measurement of the formed malonic dialdehyde (MDA) level in the rat liver homogenate. The IC50 (μM) of various concentrations of PEE in the superoxide scavenging activity in econazole (300mg/kg)-damaged rat liver homogenate were assessed by cytochrome c reduction method and compared with that of (+)-alpha-tocopherol. It could be postulated that the hepatoprotective effect of PEE may be, at least in part, due to their inhibitory ability on membrane lipid peroxidation and free radical formation or due to their free radical scavenging ability.

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Antioxidative natural product protect against econazole-induced liver injuries

Abstract

Keywords

ASJC Scopus subject areas

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