Antioxidant status following acute ischemic limb injury: A rabbit model

Jui Sheng Sun, Fung Jou Lu, Wan Ching Huang, Sheng Mou Hou, Yang Hwei Tsuang, Yi Shiong Hang

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Although ischemic injury to skeletal muscle is a matter of great clinical importance, relatively little is known about the mechanisms which determine systemic responses. One purpose of this study is to elucidate the systemic antioxidant status following an episode of acute ischemic limb injury and subsequent reperfusion. Twelve New Zealand white rabbits were used in this study. After the animals were anesthetized, an ischemic insult was created in the right hind limb for twelve hours, followed by four hours of reperfusion. Several series of blood samples were obtained. At the end of the experiment, the animals were killed and necropsies undertaken in order to evaluate the antioxidant status of various visceral organs. The results link ischemia and reperfusion injury to a significant decline in antioxidative activity in various tissues. The weakening in antioxidant status after ischemic limb injury was most pronounced in the heart tissue, followed in descending order by the spleen, skeletal muscle, lung, liver, and kidney tissue. The levels of specific antioxidants and reactive oxygen species in various organs changed significantly, and the changes were tissue specific. Endogenous radical scavenging systems were not entirely overwhelmed in most of the tissues studied. But higher levels of malondialdehyde (MDA) found in cardiac tissue suggest that the production of oxygen free radicals is accelerated by an ischemic injury. Based on the study, we believe that the cardiac tissue is particularly susceptible to the effects of ischemia and reperfusion injury. Damage to cardiac tissue is probably the major cause of mortality following acute ischemic injury in a limb.

Original languageEnglish
Pages (from-to)9-21
Number of pages13
JournalFree Radical Research
Issue number1
Publication statusPublished - 1999
Externally publishedYes


  • Antioxidant status
  • Ischemia
  • Lipid peroxidation
  • Reperfusion

ASJC Scopus subject areas

  • Biochemistry


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