Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats

Yen-Hsien Lee, Jung-Jhih Chang, Chiang-Ting Chien, Ming-Chien Yang, Hsiung-Fei Chien

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47 Citations (Scopus)


We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. © 2012 Yen-Hsien Lee et al.
Original languageEnglish
JournalExperimental Diabetes Research
Publication statusPublished - 2012
Externally publishedYes


  • ascorbic acid
  • collagen
  • poloxamer
  • sodium chloride
  • antioxidant
  • hydroxyproline
  • reactive oxygen metabolite
  • streptozocin
  • animal cell
  • animal experiment
  • animal model
  • animal tissue
  • antioxidant activity
  • apoptosis
  • article
  • collagen synthesis
  • controlled study
  • dermis
  • drug delivery system
  • drug effect
  • epidermis
  • female
  • in vitro study
  • nonhuman
  • priority journal
  • protein content
  • rat
  • skin injury
  • streptozocin diabetes
  • tissue regeneration
  • wound contraction
  • wound healing
  • animal
  • chemistry
  • experimental diabetes mellitus
  • immunohistochemistry
  • metabolism
  • methodology
  • pathology
  • pathophysiology
  • phase transition
  • skin
  • time
  • Wistar rat
  • Animals
  • Antioxidants
  • Apoptosis
  • Ascorbic Acid
  • Diabetes Mellitus, Experimental
  • Drug Delivery Systems
  • Female
  • Hydroxyproline
  • Immunohistochemistry
  • Phase Transition
  • Poloxamer
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species
  • Skin
  • Streptozocin
  • Time Factors
  • Wound Healing


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