TY - JOUR
T1 - Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats
AU - Lee, Yen-Hsien
AU - Chang, Jung-Jhih
AU - Chien, Chiang-Ting
AU - Yang, Ming-Chien
AU - Chien, Hsiung-Fei
N1 - 被引用次數：10
Export Date: 16 March 2016
通訊地址: Yang, M.-C.; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan; 電子郵件： [email protected]
化學物質/CAS: ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; collagen, 9007-34-5; poloxamer, 9003-11-6; sodium chloride, 7647-14-5; hydroxyproline, 51-35-4, 6912-67-0; streptozocin, 18883-66-4; Antioxidants; Ascorbic Acid, 50-81-7; Hydroxyproline, 51-35-4; Poloxamer, 106392-12-5; Reactive Oxygen Species; Streptozocin, 18883-66-4
製造商： Sigma Aldrich, United States
參考文獻: Singer, A.J., Clark, R.A.F., Cutaneous wound healing (1999) New England Journal of Medicine, 341 (10), pp. 738-746. , DOI 10.1056/NEJM199909023411006; Luo, J.-D., Wang, Y.-Y., Fu, W.-L., Wu, J., Chen, A.F., Gene therapy of endothelial nitric oxide synthase and manganese superoxide dismutase restores delayed wound healing in type 1 diabetic mice (2004) Circulation, 110 (16), pp. 2484-2493. , DOI 10.1161/01.CIR.0000137969.87365.05; Schorah, C.J., Downing, C., Piripitsi, A., Gallivan, L., Al-Hazaa, A.H., Sanderson, M.J., Bodenham, A., Total vitamin C, ascorbic acid, and dehydroascorbic acid concentrations in plasma of critically ill patients (1996) American Journal of Clinical Nutrition, 63 (5), pp. 760-765; Clark, R.A.F., Oxidative stress and senescent fibroblasts in non-healing wounds as potential therapeutic targets (2008) Journal of Investigative Dermatology, 128 (10), pp. 2361-2364. , 2-s2.0-51749092207 10.1038/jid.2008.257; Schneir, M., Ramamurthy, N., Golub, L., Dietary ascorbic acid normalizes diabetes-induced underhydroxylation of nascent type I collagen molecules (1985) Collagen and Related Research, 5 (5), pp. 415-422; Briganti, S., Picardo, M., Antioxidant activity, lipid peroxidation and skin diseases. What's new (2003) Journal of the European Academy of Dermatology and Venereology, 17 (6), pp. 663-669. , DOI 10.1046/j.1468-3083.2003.00751.x; Riccioni, G., Bucciarelli, T., Mancini, B., Di Ilio, C., Capra, V., D'Orazio, N., The role of the antioxidant vitamin supplementation in the prevention of cardiovascular diseases (2007) Expert Opinion on Investigational Drugs, 16 (1), pp. 25-32. , DOI 10.1517/13543784.16.1.25; Phillips, C.L., Combs, S.B., Pinnell, S.R., Effects of ascorbic acid on proliferation and collagen synthesis in relation to the donor age of human dermal fibroblasts (1994) Journal of Investigative Dermatology, 103 (2), pp. 228-232; Bickers, D.R., Athar, M., Oxidative stress in the pathogenesis of skin disease (2006) Journal of Investigative Dermatology, 126 (12), pp. 2565-2575. , DOI 10.1038/sj.jid.5700340, PII 5700340; Silvetti, A.N., An effective method of treating long-enduring wounds and ulcers by topical applications of solutions of nutrients (1981) Journal of Dermatologic Surgery and Oncology, 7 (6), pp. 501-508; Collins, N., Adding vitamin C to the wound management mix (2004) Advances in Skin & Wound Care, 17 (3), pp. 109-112. , 2-s2.0-3142628477; Bercea, M., Darie, R.N., Nita, L.E., Morariu, S., Temperature responsive gels based on Pluronic F127 and poly(vinyl alcohol) (2011) Industrial and Engineering Chemistry Research, 50 (7), pp. 4199-4206. , 2-s2.0-79953293749 10.1021/ie1024408; Schmolka, I.R., Artificial skin. I. Preparation and properties of pluronic F-127 gels for treatment of burns. (1972) Journal of Biomedical Materials Research, 6 (6), pp. 571-582. , 2-s2.0-0015420938; Bromberg, L.E., Ron, E.S., Temperature-responsive gels and thermogelling polymer matrices for protein and peptide delivery (1998) Advanced Drug Delivery Reviews, 31 (3), pp. 197-221. , DOI 10.1016/S0169-409X(97)00121-X, PII S0169409X9700121X; Sharma, P.K., Reilly, M.J., Jones, D.N., Robinson, P.M., Bhatia, S.R., The effect of pharmaceuticals on the nanoscale structure of PEO-PPO-PEO micelles (2008) Colloids and Surfaces B: Biointerfaces, 61 (1), pp. 53-60. , DOI 10.1016/j.colsurfb.2007.07.002, PII S0927776507002822; Chang, J.J., Lin, P.J., Lee, Y.H., Yang, M.C., Chien, C.T., The effect of covalent immobilization of sialic acid on the removal of lipopolysaccharide and reactive oxygen species for polyethylene terephthalate (2011) Polymer for Advanced Technologies, 22, pp. 1872-1878; Baoyong, L., Jian, Z., Denglong, C., Min, L., Evaluation of a new type of wound dressing made from recombinant spider silk protein using rat models (2010) Burns, 36 (6), pp. 891-896. , 2-s2.0-77955588339 10.1016/j.burns.2009.12.001; Yates, C.C., Whaley, D., Babu, R., Zhang, J., Krishna, P., Beckman, E., Pasculle, A.W., Wells, A., The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models (2007) Biomaterials, 28 (27), pp. 3977-3986. , DOI 10.1016/j.biomaterials.2007.05.008, PII S0142961207004048; Nemes, Z., Steinert, P.M., Bricks and mortar of the epidermal barrier (1999) Experimental and Molecular Medicine, 31 (1), pp. 5-19; Candi, E., Schmidt, R., Melino, G., The cornified envelope: A model of cell death in the skin (2005) Nature Reviews Molecular Cell Biology, 6 (4), pp. 328-340. , DOI 10.1038/nrm1619; Yamaoka, T., Takahashi, Y., Fujisato, T., Lee, C.W., Tsuji, T., Ohta, T., Murakami, A., Kimura, Y., Novel adhesion prevention membrane based on a bioresorbable copoly(ester-ether) comprised of poly-l-lactide and pluronic®: In vitro and in vivo evaluations (2001) Journal of Biomedical Materials Research, 54 (4), pp. 470-479. , DOI 10.1002/1097-4636(20010315)54:4<470::AID-JBM20>3.0.CO;2-X; Oh, S.H., Kim, J.K., Song, K.S., Noh, S.M., Ghil, S.H., Yuk, S.H., Lee, J.H., Prevention of postsurgical tissue adhesion by anti-inflammatory drug-loaded Pluronic mixtures with sol-gel transition behavior (2005) Journal of Biomedical Materials Research - Part A, 72 (3), pp. 306-316. , DOI 10.1002/jbm.a.30239; Hokett, S.D., Cuenin, M.F., O'Neal, R.B., Brennan, W.A., Strong, S.L., Runner, R.R., McPherson, J.C., Van Dyke, T.E., Pluronic polyol effects on human gingival fibroblast attachment and growth (2000) Journal of Periodontology, 71 (5), pp. 803-809; Fowler, E.B., Cuenin, M.F., Hokett, S.D., Peacock, M.E., McPherson III, J.C., Dirksen, T.R., Sharawy, M., Billman, M.A., Evaluation of pluronic polyols as carriers for grafting materials: Study in rat calvaria defects (2002) Journal of Periodontology, 73 (2), pp. 191-197. , DOI 10.1902/jop.2002.73.2.191; Khalil, E.A., Afifi, F.U., Al-Hussaini, M., Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in Pluronic F127 using mice (Mus musculus) (2007) Journal of Ethnopharmacology, 109 (1), pp. 104-112. , DOI 10.1016/j.jep.2006.07.010, PII S0378874106003473; Abd-El-Aleem, S.A., Ferguson, M.W.J., Appleton, I., Kairsingh, S., Jude, E.B., Jones, K., McCollum, C.N., Ireland, G.W., Expression of nitric oxide synthase isoforms and arginase in normal human skin and chronic venous leg ulcers (2000) Journal of Pathology, 191 (4), pp. 434-442; Rojkind, M., Dominguez-Rosales, J.-A., Nieto, N., Greenwel, P., Role of hydrogen peroxide and oxidative stress in healing responses (2002) Cellular and Molecular Life Sciences, 59 (11), pp. 1872-1891. , DOI 10.1007/PL00012511; Moseley, R., Hilton, J.R., Waddington, R.J., Harding, K.G., Stephens, P., Thomas, D.W., Comparison of oxidative stress biomarker profiles between acute and chronic wound environments (2004) Wound Repair and Regeneration, 12 (4), pp. 419-429. , DOI 10.1111/j.1067-1927.2004.12406.x; Rasik, A.M., Shukla, A., Antioxidant status in delayed healing type of wounds (2000) International Journal of Experimental Pathology, 81 (4), pp. 257-263. , 2-s2.0-0033864199 10.1046/j.1365-2613.2000.00158.x; Gopinath, D., Ahmed, M.R., Gomathi, K., Chitra, K., Sehgal, P.K., Jayakumar, R., Dermal wound healing processes with curcumin incorporated collagen films (2004) Biomaterials, 25 (10), pp. 1911-1917. , DOI 10.1016/S0142-9612(03)00625-2; Khanna, S., Roy, S., Bagchi, D., Bagchi, M., Sen, C.K., Upregulation of oxidant-induced VEGF expression in cultured keratinocytes by a grape seed proanthocyanidin extract (2001) Free Radical Biology and Medicine, 31 (1), pp. 38-42. , DOI 10.1016/S0891-5849(01)00544-5, PII S0891584901005445; Fadini, G.P., Albiero, M., Menegazzo, L., Boscaro, E., Pagnin, E., Iori, E., Cosma, C., Avogaro, A., The redox enzyme p66Shc contributes to diabetes and ischemia-induced delay in cutaneous wound healing (2010) Diabetes, 59 (9), pp. 2306-2314. , 2-s2.0-77956379343 10.2337/db09-1727; Al-Mashat, H.A., Kandru, S., Liu, R., Behl, Y., Desta, T., Graves, D.T., Diabetes enhances mRNA levels of proapoptotic genes and caspase activity, which contribute to impaired healing (2006) Diabetes, 55 (2), pp. 487-495. , http://diabetes.diabetesjournals.org/cgi/reprint/55/2/487, DOI 10.2337/diabetes.55.02.06.db05-1201
PY - 2012
Y1 - 2012
N2 - We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. © 2012 Yen-Hsien Lee et al.
AB - We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer. © 2012 Yen-Hsien Lee et al.
KW - ascorbic acid
KW - collagen
KW - poloxamer
KW - sodium chloride
KW - antioxidant
KW - hydroxyproline
KW - reactive oxygen metabolite
KW - streptozocin
KW - animal cell
KW - animal experiment
KW - animal model
KW - animal tissue
KW - antioxidant activity
KW - apoptosis
KW - article
KW - collagen synthesis
KW - controlled study
KW - dermis
KW - drug delivery system
KW - drug effect
KW - epidermis
KW - female
KW - in vitro study
KW - nonhuman
KW - priority journal
KW - protein content
KW - rat
KW - skin injury
KW - streptozocin diabetes
KW - tissue regeneration
KW - wound contraction
KW - wound healing
KW - animal
KW - chemistry
KW - experimental diabetes mellitus
KW - immunohistochemistry
KW - metabolism
KW - methodology
KW - pathology
KW - pathophysiology
KW - phase transition
KW - skin
KW - time
KW - Wistar rat
KW - Animals
KW - Antioxidants
KW - Apoptosis
KW - Ascorbic Acid
KW - Diabetes Mellitus, Experimental
KW - Drug Delivery Systems
KW - Female
KW - Hydroxyproline
KW - Immunohistochemistry
KW - Phase Transition
KW - Poloxamer
KW - Rats
KW - Rats, Wistar
KW - Reactive Oxygen Species
KW - Skin
KW - Streptozocin
KW - Time Factors
KW - Wound Healing
U2 - 10.1155/2012/504693
DO - 10.1155/2012/504693
M3 - Article
SN - 1687-5214
VL - 2012
JO - Experimental Diabetes Research
JF - Experimental Diabetes Research
ER -
