TY - JOUR
T1 - Antioxidant effect of hydralazine on retinal pigment epithelial cells and its potential use in the therapy of age-related macular degeneration
AU - Cheng, Yu Wen
AU - Chiou, George C Y
PY - 2008/6/25
Y1 - 2008/6/25
N2 - • Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).
AB - • Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).
KW - Age-related macular degeneration (AMD)
KW - Reactive oxygen species (ROS)
KW - Tea-butyl hydroxyperoxide (t-BHP)
KW - Age-related macular degeneration (AMD)
KW - Reactive oxygen species (ROS)
KW - Tea-butyl hydroxyperoxide (t-BHP)
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M3 - Article
AN - SCOPUS:47249105549
SN - 1672-5123
VL - 8
SP - 1059
EP - 1064
JO - International Journal of Ophthalmology
JF - International Journal of Ophthalmology
IS - 6
ER -