Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate

Yuh Hwa Liu; Wen Li Liang; Chi Ching Lee; Yow Fu Tsai; Wen Chi Hou

doi:10.1016/j.foodchem.2011.04.094

Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate

Yuh Hwa Liu, Wen Li Liang, Chi Ching Lee, Yow Fu Tsai, Wen Chi Hou

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The antioxidant and inhibitory activities of glucuronic acid (GluA) and its synthesized 1-methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH) against semicarbazide-sensitive amine oxidase (SSAO) were investigated. The scavenging activities of GluA-NHOH against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radicals (half-inhibition concentration [IC₅₀], 152 μM) and hydroxyl radicals (IC₅₀, 70 nM) were determined. The protective activities of GluA-NHOH against hydroxyl radical-mediated calf thymus DNA damage and peroxynitrite-mediated dihydrorhodamine oxidation were also investigated. GluA-NHOH, but not GluA, exhibited dose-dependent protective activities. GluA-NHOH, but not GluA, effectively inhibited bovine SSAO activities in a dose-dependent manner, and was further confirmed by AO activity stains on an acrylamide gel. GluA-NHOH showed mixed noncompetitive inhibition of bovine SSAO with respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). The Vmax′ and Km′ values were reduced in the presence of GluA-NHOH (0.04 mM).

Original language	English
Pages (from-to)	423-428
Number of pages	6
Journal	Food Chemistry
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2011.04.094
Publication status	Published - Nov 15 2011

Keywords

1-Methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH)
Antioxidant activity
Electron spin resonance (ESR)

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2011.04.094

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title = "Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate",

abstract = "The antioxidant and inhibitory activities of glucuronic acid (GluA) and its synthesized 1-methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH) against semicarbazide-sensitive amine oxidase (SSAO) were investigated. The scavenging activities of GluA-NHOH against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radicals (half-inhibition concentration [IC50], 152 μM) and hydroxyl radicals (IC50, 70 nM) were determined. The protective activities of GluA-NHOH against hydroxyl radical-mediated calf thymus DNA damage and peroxynitrite-mediated dihydrorhodamine oxidation were also investigated. GluA-NHOH, but not GluA, exhibited dose-dependent protective activities. GluA-NHOH, but not GluA, effectively inhibited bovine SSAO activities in a dose-dependent manner, and was further confirmed by AO activity stains on an acrylamide gel. GluA-NHOH showed mixed noncompetitive inhibition of bovine SSAO with respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). The Vmax′ and Km′ values were reduced in the presence of GluA-NHOH (0.04 mM).",

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author = "Liu, {Yuh Hwa} and Liang, {Wen Li} and Lee, {Chi Ching} and Tsai, {Yow Fu} and Hou, {Wen Chi}",

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AU - Liang, Wen Li

AU - Lee, Chi Ching

AU - Tsai, Yow Fu

AU - Hou, Wen Chi

PY - 2011/11/15

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N2 - The antioxidant and inhibitory activities of glucuronic acid (GluA) and its synthesized 1-methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH) against semicarbazide-sensitive amine oxidase (SSAO) were investigated. The scavenging activities of GluA-NHOH against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radicals (half-inhibition concentration [IC50], 152 μM) and hydroxyl radicals (IC50, 70 nM) were determined. The protective activities of GluA-NHOH against hydroxyl radical-mediated calf thymus DNA damage and peroxynitrite-mediated dihydrorhodamine oxidation were also investigated. GluA-NHOH, but not GluA, exhibited dose-dependent protective activities. GluA-NHOH, but not GluA, effectively inhibited bovine SSAO activities in a dose-dependent manner, and was further confirmed by AO activity stains on an acrylamide gel. GluA-NHOH showed mixed noncompetitive inhibition of bovine SSAO with respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). The Vmax′ and Km′ values were reduced in the presence of GluA-NHOH (0.04 mM).

AB - The antioxidant and inhibitory activities of glucuronic acid (GluA) and its synthesized 1-methoxyl-6-glucuronic acid hydroxamate (GluA-NHOH) against semicarbazide-sensitive amine oxidase (SSAO) were investigated. The scavenging activities of GluA-NHOH against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radicals (half-inhibition concentration [IC50], 152 μM) and hydroxyl radicals (IC50, 70 nM) were determined. The protective activities of GluA-NHOH against hydroxyl radical-mediated calf thymus DNA damage and peroxynitrite-mediated dihydrorhodamine oxidation were also investigated. GluA-NHOH, but not GluA, exhibited dose-dependent protective activities. GluA-NHOH, but not GluA, effectively inhibited bovine SSAO activities in a dose-dependent manner, and was further confirmed by AO activity stains on an acrylamide gel. GluA-NHOH showed mixed noncompetitive inhibition of bovine SSAO with respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). The Vmax′ and Km′ values were reduced in the presence of GluA-NHOH (0.04 mM).

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Antioxidant and semicarbazide-sensitive amine oxidase inhibitory activities of glucuronic acid hydroxamate

Abstract

Keywords

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