TY - JOUR
T1 - Antioxidant activities of ethanolic extracts from the twigs of Cinnamomum osmophloeum
AU - Chua, Meng Thong
AU - Tung, Yu Tang
AU - Chang, Shang Tzen
N1 - Funding Information:
We thank the Forestry Bureau of Council of Agriculture for the financial support (96AS-12.2.2-FB-e1) and Ms. Shou-Ling Huang (Department of Chemistry, National Taiwan University) for NMR spectral analyses.
PY - 2008/4
Y1 - 2008/4
N2 - Cinnamomum osmophloeum Kaneh. is an indigenous tree species in Taiwan. In this study, phytochemical characteristics and antioxidant activities of ethanolic extracts from the twigs of C. osmophloeum were investigated for the first time. The extracts were obtained successively using liquid-liquid partition to yield n-hexane (Hex), ethyl acetate (EtOAc), n-butanol (BuOH) and water fractions. The antioxidant activities of C. osmophloeum twig extracts were examined using various antioxidant assays, such as DPPH, NBT, reducing power, lipid peroxidation using mouse brain homogenates, metal chelating ability, and photochemiluminescence (PCL). In addition, total phenolic contents were also determined. Results revealed that the BuOH fraction exhibited the best performance in DPPH assay, NBT assay, reducing power assay and lipid peroxidation using mouse brain homogenates assay. Furthermore, the BuOH fraction has the highest total phenolic contents (496.7 mg of GAE/g). Consequently, kaempferol-7-O-rhamnoside was also isolated from the antioxidative BuOH fraction and its activity was also confirmed. These results demonstrated that ethanolic extracts of C. osmophloeum twigs have excellent antioxidant activities and thus it has great potential as a source for natural health products.
AB - Cinnamomum osmophloeum Kaneh. is an indigenous tree species in Taiwan. In this study, phytochemical characteristics and antioxidant activities of ethanolic extracts from the twigs of C. osmophloeum were investigated for the first time. The extracts were obtained successively using liquid-liquid partition to yield n-hexane (Hex), ethyl acetate (EtOAc), n-butanol (BuOH) and water fractions. The antioxidant activities of C. osmophloeum twig extracts were examined using various antioxidant assays, such as DPPH, NBT, reducing power, lipid peroxidation using mouse brain homogenates, metal chelating ability, and photochemiluminescence (PCL). In addition, total phenolic contents were also determined. Results revealed that the BuOH fraction exhibited the best performance in DPPH assay, NBT assay, reducing power assay and lipid peroxidation using mouse brain homogenates assay. Furthermore, the BuOH fraction has the highest total phenolic contents (496.7 mg of GAE/g). Consequently, kaempferol-7-O-rhamnoside was also isolated from the antioxidative BuOH fraction and its activity was also confirmed. These results demonstrated that ethanolic extracts of C. osmophloeum twigs have excellent antioxidant activities and thus it has great potential as a source for natural health products.
KW - Antioxidant activity
KW - Cinnamomum osmophloeum
KW - Extracts
KW - Photochemiluminescence assay
KW - Twigs
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U2 - 10.1016/j.biortech.2007.03.020
DO - 10.1016/j.biortech.2007.03.020
M3 - Article
C2 - 17478090
AN - SCOPUS:37549035820
SN - 0960-8524
VL - 99
SP - 1918
EP - 1925
JO - Agricultural Wastes
JF - Agricultural Wastes
IS - 6
ER -