TY - JOUR
T1 - Antimutagenic constituents of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity
AU - Chen, Huang Hui
AU - Chiang, Wenchang
AU - Chang, Jang Yang
AU - Chien, Ya Lin
AU - Lee, Ching Kuo
AU - Liu, Ko Jiunn
AU - Cheng, Yen Ting
AU - Chen, Ting Fang
AU - Kuo, Yueh Hsiung
AU - Kuo, Ching Chuan
PY - 2011/6/22
Y1 - 2011/6/22
N2 - Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans- coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.
AB - Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans- coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.
KW - ARE
KW - adlay
KW - antimutagenic constituents
KW - cancer chemoprevention
KW - kinase signal
KW - traditional Chinese medicine
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U2 - 10.1021/jf200539r
DO - 10.1021/jf200539r
M3 - Article
C2 - 21561091
AN - SCOPUS:79959224748
SN - 0021-8561
VL - 59
SP - 6444
EP - 6452
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 12
ER -