Antifibrotic effect of bletilla striata polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy

Hsuan Yu Chen; Tzu Chieh Lin; Chih Yung Chiang; Shiuan Li Wey; Feng Huei Lin; Kai Chiang Yang; Chih Hao Chang; Ming Hsiao Hu

doi:10.3390/polym13132129

Antifibrotic effect of bletilla striata polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy

Hsuan Yu Chen, Tzu Chieh Lin, Chih Yung Chiang, Shiuan Li Wey, Feng Huei Lin, Kai Chiang Yang, Chih Hao Chang, Ming Hsiao Hu

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable car-boxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompati-bility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expan-sion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.

Original language	English
Article number	2129
Journal	Polymers
Volume	13
Issue number	13
DOIs	https://doi.org/10.3390/polym13132129
Publication status	Published - Jul 2021

Keywords

Bletilla striata
Carboxymethyl cellulose
Epidural fibrosis
Laminectomy
Resveratrol

ASJC Scopus subject areas

General Chemistry
Polymers and Plastics

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10.3390/polym13132129

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title = "Antifibrotic effect of bletilla striata polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy",

abstract = "The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable car-boxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompati-bility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expan-sion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.",

keywords = "Bletilla striata, Carboxymethyl cellulose, Epidural fibrosis, Laminectomy, Resveratrol",

author = "Chen, {Hsuan Yu} and Lin, {Tzu Chieh} and Chiang, {Chih Yung} and Wey, {Shiuan Li} and Lin, {Feng Huei} and Yang, {Kai Chiang} and Chang, {Chih Hao} and Hu, {Ming Hsiao}",

note = "Funding Information: Funding: We are grateful for funding from National Taiwan University Hospital. Funding Information: Acknowledgments: Authors would like to thank the Department of Biomedical Engineering, National Taiwan University for their financial support and providing necessary materials for this study. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = jul,

doi = "10.3390/polym13132129",

language = "English",

volume = "13",

journal = "Polymers",

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AU - Chen, Hsuan Yu

AU - Lin, Tzu Chieh

AU - Chiang, Chih Yung

AU - Wey, Shiuan Li

AU - Lin, Feng Huei

AU - Yang, Kai Chiang

AU - Chang, Chih Hao

AU - Hu, Ming Hsiao

N1 - Funding Information: Funding: We are grateful for funding from National Taiwan University Hospital. Funding Information: Acknowledgments: Authors would like to thank the Department of Biomedical Engineering, National Taiwan University for their financial support and providing necessary materials for this study. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/7

Y1 - 2021/7

N2 - The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable car-boxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompati-bility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expan-sion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.

AB - The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable car-boxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompati-bility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expan-sion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.

KW - Bletilla striata

KW - Carboxymethyl cellulose

KW - Epidural fibrosis

KW - Laminectomy

KW - Resveratrol

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DO - 10.3390/polym13132129

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Antifibrotic effect of bletilla striata polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy

Abstract

Keywords

ASJC Scopus subject areas

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